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首页> 外文期刊>Acta biomaterialia >Fabrication of patterned cell co-cultures on albumin-based substrate: applications for microfluidic devices.
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Fabrication of patterned cell co-cultures on albumin-based substrate: applications for microfluidic devices.

机译：在白蛋白基底物上的图案化细胞共培养的制造：微流体装置的应用。

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摘要

A surface coated with cross-linked albumin film resists the adhesion of cells, and subsequent exposure to UV irradiation or electrostatic adsorption of a cationic polymer switches the surface from non-adherent to adherent. Taking advantage of this unique property of cross-linked albumin, the authors fabricated patterned cell co-cultures with desired patterns and cell types. In this scheme, the cell-adherent region was initially created in the cell-non-adhesive albumin substrate, on which a first cell type was attached. Subsequently, the remaining region was also changed to adherent for the attachment of secondary cells in the same manner, thereby allowing distinctly localized co-cultures. As a proof of concept demonstration of the feasibility of this approach, a patterned co-culture of Neuro-2a cells with L929 cells was successfully prepared on the substrate. Furthermore, combining this technique with a microfluidic technique, a micropatterned co-culture of PA6 cells with 3T3 fibroblasts was created inside microfluidic devices. This approach could potentially be a useful tool for fundamental investigations of cell-cell interactions and for tissue engineering applications.
机译：涂有交联白蛋白膜的表面抵抗细胞的粘附性，随后暴露于阳离子聚合物的UV照射或静电吸附，从非粘附物切换表面。利用这种交联白蛋白的这种独特性，作者用所需的图案和细胞类型制造了图案化的细胞共培养物。在该方案中，最初在细胞 - 非粘合白蛋白基质中形成细胞粘附区域，其中附着第一细胞类型。随后，还改变剩余区域以以相同的方式粘附次要细胞，从而允许明显局部化的共培养物。作为这种方法可行性概念证明的证据，在基材上成功地制备了具有L929细胞的神经2A细胞的图案化共培养。此外，将该技术与微流体技术相结合，在微流体装置内产生具有3T3成纤维细胞的PA6细胞的微透明理由的共培养。这种方法可能是细胞 - 细胞相互作用和组织工程应用的基本研究的有用工具。

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  • 来源
    《Acta biomaterialia》 |2010年第2期|共8页
  • 作者

    Yamazoe H; Okuyama T; Suzuki;

  • 作者单位

    NanoBio Medical Technology Group Nanotechnology Research Institute National Institute of Advanced Industrial Science and Technology (AIST) Central 4-1 Tsukuba Ibaraki Japan.;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物医学工程;
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