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首页> 外文期刊>Acta Chiropterologica >Effect of sample preservation methods on the viability of Geomyces destructans, the fungus associated with white-nose syndrome in bats
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Effect of sample preservation methods on the viability of Geomyces destructans, the fungus associated with white-nose syndrome in bats

机译：样品保存方法对蝙蝠白鼻综合症所致真菌-毁灭性霉菌活力的影响

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摘要

Geomyces destructans is a fungus directly associated with white-nose syndrome (WNS), a recently discovered disease that has caused the death of over one million bats in North America and therefore has prompted intense scientific investigation into its biology. If precautions are not taken, the spread of G. destructans by human transportation for scientific study may be a substantial threat to bat populations. We investigated the viability of G. destructans after being exposed to commonly used DNA/RNA preservation methods. Our first experiment revealed that G. destructans is able to germinate after an eight day storage period in RNA later or kept dry but could not germinate after storage in 70% or absolute ethanol for the same time period. Storing G. destructans samples at different temperatures did not substantially affect the results. In a second experiment, we showed that G. destructans conidia were only killed after being stored in 70% ethanol for a minimum of 24 hours while only 30 minutes were necessary when stored in absolute ethanol. Our results suggest that the DNA/RNA preservation method has an important impact on the ability of G. destructans to remain viable and should therefore be considered before samples are transported, especially to regions where the fungus has not yet been documented. Our results also strongly advocate the use of high concentrations (i.e., absolute) ethanol over 70% ethanol to rapidly kill G. destructans.
机译：毁灭性霉菌是一种与白鼻综合症（WNS）直接相关的真菌，这种病是最近发现的一种疾病，已导致北美超过一百万只蝙蝠死亡，因此促使对其生物学进行深入的科学调查。如果不采取预防措施，人类为了科学研究而运输的De。G. destructans可能会严重威胁蝙蝠种群。在暴露于常用的DNA / RNA保存方法后，我们研究了G. destructans的生存能力。我们的第一个实验表明，毁灭性链球菌能够在RNA中储存8天后发芽，或者保持干燥，但是在70％的乙醇或无水乙醇中储存相同的时间后就不会发芽。在不同温度下储存毁灭性链霉菌样品不会对结果产生实质性影响。在第二个实验中，我们显示了仅在70％乙醇中储存至少24小时后才杀死G. destructans分生孢子，而在无水乙醇中只需要30分钟。我们的结果表明，DNA / RNA的保存方法对变形链球菌保持活力的能力具有重要影响，因此，在运输样品之前，尤其是尚未记录到真菌的区域之前，应考虑使用这种方法。我们的结果还强烈主张使用高浓度（即，绝对）乙醇而不是70％乙醇来快速杀死G. destructans。

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