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首页> 外文期刊>Acta biomaterialia >Characterization and in vitro cytocompatibility of piezoelectric electrospun scaffolds.
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Characterization and in vitro cytocompatibility of piezoelectric electrospun scaffolds.

机译：压电电纺支架的表征和体外细胞相容性。

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Previous studies have shown that electrical charges influence cell behavior (e.g. enhancement of nerve regeneration, cell adhesion, cell morphology). Thus, piezoelectric scaffolds might be useful for various tissue engineering applications. Fibrous scaffolds were successfully fabricated from permanent piezoelectric poly(vinylidene fluoride-trifluoroethylene) (PVDF-TrFE) by the electrospinning technique. Scanning electron microscopy and capillary flow analyses verified that the fiber mats had an average fiber diameter of 970 +/- 480 nm and a mean pore diameter of 1.7 microm, respectively. Thermally stimulated depolarization current spectroscopy measurements confirmed the piezoelectric property of the PVDF-TrFE fibrous scaffolds by the generation of a spontaneous current with the increase in temperature in the absence of an electric field, which was not detected in the unprocessed PVDF-TrFE powder. Differential scanning calorimetry, thermogravimetric analysis, X-ray diffraction and Fourier transform infrared spectroscopy results showed that the electrospinning process increased the crystallinity and presence of the polar, beta-phase crystal compared with the unprocessed powder. Confocal fluorescence microscopy and a cell proliferation assay demonstrated spreading and increased cell numbers (human skin fibroblasts) over time on PVDF-TrFE scaffolds, which was comparable with tissue culture polystyrene. The relative quantity of gene expression for focal adhesion proteins (measured by real-time RT-PCR) increased in the following order: paxillin < vinculin < focal adhesion kinase < talin. However, no differences could be seen among the TCPS surface and the fibrous scaffolds. Future studies will focus on possible applications of these cytocompatible PVDF-TrFE scaffolds in the field of regenerative medicine.
机译：先前的研究表明，电荷会影响细胞行为（例如，增强神经再生，细胞粘附，细胞形态）。因此，压电支架对于各种组织工程应用可能是有用的。通过静电纺丝技术成功地由永久性压电聚偏二氟乙烯-三氟乙烯（PVDF-TrFE）制成了纤维支架。扫描电子显微镜和毛细管流动分析证实，纤维垫的平均纤维直径分别为970 +/- 480nm和平均孔径为1.7μm。热激发的去极化电流光谱学测量证实了在不存在电场的情况下，随着温度的升高，自发电流随温度的升高而产生自发电流，从而证实了PVDF-TrFE纤维支架的压电特性，这在未处理的PVDF-TrFE粉末中未检测到。差示扫描量热法，热重分析，X射线衍射和傅里叶变换红外光谱结果表明，与未处理的粉末相比，电纺丝过程增加了极性和β相晶体的结晶度和存在。共聚焦荧光显微镜和细胞增殖测定表明，PVDF-TrFE支架上的散布和增加的细胞数量（人皮肤成纤维细胞）随时间推移而增加，这与组织培养的聚苯乙烯相当。粘着斑粘附蛋白基因表达的相对数量（通过实时RT-PCR测量）按以下顺序增加：paxillin <纽蛋白<粘着斑激酶

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