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首页> 外文期刊>Acta biomaterialia >Remarkably enhanced stability and function of core/shell nanoparticles composed of a lecithin core and a pluronic shell layer by photo-crosslinking the shell layer: in vitro and in vivo study.
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Remarkably enhanced stability and function of core/shell nanoparticles composed of a lecithin core and a pluronic shell layer by photo-crosslinking the shell layer: in vitro and in vivo study.

机译：通过卵交联壳层，显着增强了由卵磷脂核和普鲁尼克壳层组成的核/壳纳米颗粒的稳定性和功能：体外和体内研究。

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A core/shell nanoparticle system with a lecithin core and a pluronic shell has been previously reported, and it was shown to act as an effective sustained release system for positively charged proteins. Here, to provide improved stability of the core/shell nanoparticle system in a physiological environment, we prepared the core/shell nanoparticle system with a photo-crosslinked shell layer by using a lecithin liposome as the core and pluronic F 127 diacrylate (DA-PF 127) as the shell layer. The DA-PF 127 was then photo-polymerized. Compared with a purely physical system, chemical crosslinking of the shell layer resulted not only in significantly increased structural stability of the core/shell nanoparticles in both an organic co-solvent and in serum but also several remarkably enhanced functioning as a protein delivery system. First, the chemically crosslinked systems were resuspended in aqueous solution after lyophilization without using a cryo-protectant. Second, target proteins were efficiently loaded into the nanoparticles by simple co-incubation in aqueous solution at a low temperature (4 degrees C) and the dried powder form of the protein-loaded nanoparticles was obtained. The loading capacity of the system was increased by more than 10 times compared with that of a purely physical system. Most importantly, the chemically crosslinked system showed more sustained release of the loaded proteins, and the release rate was not noticeably affected by the presence of serum proteins, whereas sustained release of loaded vascular endothelial growth factor (VEGF) in a purely physical system was greatly reduced by serum proteins. In an in vivo corneal angiogenesis assay the chemically crosslinked system loaded with VEGF resulted in more efficient new blood vessel formation than the physical system.
机译：先前已经报道了具有卵磷脂核和普朗尼克壳的核/壳纳米粒子系统，并且它被证明可以作为带正电荷蛋白质的有效缓释系统。在这里，为了提高核/壳纳米颗粒系统在生理环境中的稳定性，我们通过使用卵磷脂脂质体作为核芯和普卢尼克F 127二丙烯酸酯（DA-PF），制备了具有光交联壳层的核/壳纳米颗粒系统。 127）作为外壳层。然后将DA-PF 127光聚合。与纯物理系统相比，壳层的化学交联不仅导致核/壳纳米颗粒在有机助溶剂和血清中的结构稳定性显着提高，而且还显着增强了作为蛋白质传递系统的功能。首先，在不使用冷冻保护剂的情况下，将冻干后的化学交联体系重悬于水溶液中。其次，通过在低温（4摄氏度）下在水溶液中简单共孵育，将目标蛋白有效地负载到纳米颗粒中，并获得了负载蛋白的纳米颗粒的干燥粉末形式。与纯物理系统相比，系统的负载能力增加了十倍以上。最重要的是，化学交联系统显示了负载蛋白的更持续释放，并且释放速度不受血清蛋白的存在的显着影响，而在纯物理系统中负载血管内皮生长因子（VEGF）的持续释放却非常明显。降低血清蛋白。在体内角膜血管生成测定中，负载有VEGF的化学交联系统比物理系统更有效地形成了新血管。

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