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首页> 外文期刊>Acta biomaterialia >The odontogenic differentiation of human dental pulp stem cells on nanofibrous poly(l-lactic acid) scaffolds in vitro and in vivo.
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The odontogenic differentiation of human dental pulp stem cells on nanofibrous poly(l-lactic acid) scaffolds in vitro and in vivo.

机译：人类牙髓干细胞在纳米纤维聚（1-乳酸）支架上的体外和体内牙源性分化。

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The aim of this study was to investigate the odontogenic differentiation of human dental pulp stem cells (DPSCs) on nanofibrous (NF)-poly(l-lactic acid) (PLLA) scaffolds in vitro and in vivo. Highly porous NF-PLLA scaffolds which mimic the architecture of collagen type I fibers were fabricated by the combination of a phase-separation technique and a porogen-leaching method. The human DPSCs were then seeded onto the scaffolds and cultured in different media for odontogenic differentiation: "Control" medium without supplements; "DXM" medium containing 10(-8)M dexamethasone (DXM), 50 microgml(-1) ascorbic acid and 5mM beta-glycerophosphate; "BMP-7+DXM" medium containing 10(-8)M DXM, 50 microgml(-1) ascorbic acid, 5mM beta-glycerophosphate plus 50 ngml(-1) bone morphogenetic protein 7 (BMP-7). For odontogenic differentiation study in vitro, alkaline phosphatase activity quantification, reverse transcription polymerase chain reaction, scanning electron microscopy, von Kossa staining and calcium content quantification were carried out. While both "DXM" medium and "BMP-7+DXM" medium induced the DPSCs to odontoblast-like cells, the "BMP-7+DXM" medium had greater inducing capacity than the "DXM" medium. Consistent with the in vitro studies, the "BMP-7+DXM" group presented more extracellular matrix and hard tissue formation than the "DXM" group after 8 weeks of ectopic implantation in nude mice. Differentiation of DPSCs into odontoblast-like cells was identified by the positive immunohistochemical staining for dentin sialoprotein. In conclusion, odontogenic differentiation of DPSCs can be achieved on NF-PLLA scaffolds both in vitro and in vivo; the combination of BMP-7 and DXM induced the odontogenic differentiation more effectively than DXM alone. The NF-PLLA scaffold and the combined odontogenic inductive factors provide excellent environment for DPSCs to regenerate dental pulp and dentin.
机译：这项研究的目的是在体外和体内研究人类牙髓干细胞（DPSCs）在纳米纤维（NF）-聚（1-乳酸）（PLLA）支架上的牙源性分化。通过相分离技术和成孔剂浸出法的组合，制造了模拟I型胶原纤维结构的高度多孔的NF-PLLA支架。然后将人DPSCs接种到支架上，并在不同的培养基中培养以进行牙源性分化。含有10（-8）M地塞米松（DXM），50微克（-1）抗坏血酸和5mMβ-甘油磷酸盐的“ DXM”培养基;包含10（-8）M DXM，50 microgml（-1）抗坏血酸，5mMβ-甘油磷酸酯和50 ngml（-1）骨形态发生蛋白7（BMP-7）的“ BMP-7 + DXM”培养基。为了进行牙源性分化研究，进行了碱性磷酸酶活性定量，逆转录聚合酶链反应，扫描电子显微镜，von Kossa染色和钙含量定量。尽管“ DXM”培养基和“ BMP-7 + DXM”培养基均诱导DPSCs进入成牙本质细胞样细胞，但“ BMP-7 + DXM”培养基的诱导能力比“ DXM”培养基大。与体外研究一致，裸鼠异位植入8周后，“ BMP-7 + DXM”组比“ DXM”组表现出更多的细胞外基质和硬组织形成。通过牙本质唾液蛋白的阳性免疫组织化学染色鉴定了DPSCs分化为成牙本质细胞样细胞。总之，在体外和体内均可在NF-PLLA支架上实现DPSC的牙源性分化。 BMP-7和DXM的组合比单独使用DXM更有效地诱导牙源性分化。 NF-PLLA支架和结合的牙源性诱导因子为DPSC再生牙髓和牙本质提供了极好的环境。

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