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首页> 外文期刊>Acta biomaterialia >Active fluctuation in the cortical cytoskeleton observed by high-speed live-cell scanning probe microscopy.
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Active fluctuation in the cortical cytoskeleton observed by high-speed live-cell scanning probe microscopy.

机译：高速活细胞扫描探针显微镜观察到的皮质细胞骨架的主动波动。

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摘要

We investigated the dynamics of the cortical cytoskeleton in living cells by analyzing the motion of the endogenous components of the cytoskeleton using scanning probe microscopy (SPM). We performed molecular characterization of the microgranules visualized by SPM in living cells and analyzed the motion of these microgranules via particle tracking. Simultaneous SPM and epifluorescence microscopy observations showed that the microgranules recruited not only actin but also cortactin, which can bind to actin filaments. This indicates condensation of actin filaments at microgranules, leading us to identify them as cytoskeletal microdomains analysis showed that these cytoskeletal microdomains exhibit random walk-like diffusive fluctuations over a timescale of seconds. Inhibition of the molecular motor myosin II, which drives actin filaments, led to subdiffusive fluctuations of the microdomains. These results can be explained by longitudinal sliding of actin filaments stochastically driven by myosin II and the bending motion of the actin filaments in the absence of sliding. Analysis of the cytoskeletal microdomains thus revealed the intrinsic dynamics of the cortical cytoskeleton.
机译：我们通过使用扫描探针显微镜（SPM）分析细胞骨架的内源性成分的运动，研究了活细胞中皮质细胞骨架的动力学。我们对SPM在活细胞中观察到的微粒进行了分子表征，并通过粒子跟踪分析了这些微粒的运动。同时进行的SPM和落射荧光显微镜观察表明，微粒不仅募集了肌动蛋白，还募集了可以与肌动蛋白细丝结合的皮质激素。这表明肌动蛋白丝在微粒处凝结，导致我们将其鉴定为细胞骨架微区分析表明，这些细胞骨架微区在数秒的时间内显示出随机的步行样扩散波动。驱动肌动蛋白丝的分子运动肌球蛋白II的抑制导致微域的亚扩散性波动。这些结果可以通过肌球蛋白II随机驱动的肌动蛋白丝的纵向滑动和肌动蛋白丝在不存在滑动的情况下的弯曲运动来解释。因此，对细胞骨架微区的分析揭示了皮质细胞骨架的内在动力学。

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