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首页> 外文期刊>Acta biomaterialia >Increasing capillary diameter and the incorporation of gelatin enhance axon outgrowth in alginate-based anisotropic hydrogels.
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Increasing capillary diameter and the incorporation of gelatin enhance axon outgrowth in alginate-based anisotropic hydrogels.

机译：在基于藻酸盐的各向异性水凝胶中，增加毛细管直径和加入明胶可增强轴突的生长。

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Substantial recovery of function following peripheral and central nervous system (CNS) injury critically depends on longitudinally directed axon regeneration across the injury site, which requires a mechanical guidance providing scaffold. We have previously shown that anisotropic alginate-based hydrogels with a defined capillary diameter (25 mum), which form via a self-organizing process driven by unidirectional diffusion of divalent cations into sodium alginate sols, promoted longitudinally oriented elongation of CNS axons in vitro and in vivo. In the present study the influence of various capillary diameters and the incorporation of gelatin to promote directed axon outgrowth and Schwann cell migration were assessed in a dorsal root ganglion outgrowth assay in vitro. Superimposing an alginate sol with Cu(2+), Sr(2+), or Zn(2+) ion containing solutions allowed the creation of hydrogels with capillaries 18, 25 and 55 mum in diameter, respectively. Axon outgrowth and Schwann cell migration were analyzed in terms of axon length/density and Schwann cell density within the capillary structures. Axon ingrowth into capillary hydrogels, which was always accompanied by Schwann cells, was enhanced with increasing capillary diameter. The incorporation of gelatin did not influence overall axon density, but promoted the length of axon outgrowth within the hydrogels. The longitudinal orientation of axons decreased in wider capillaries, which suggests that medium-sized capillaries are the optimal substrate to elicit substantial axon growth and longitudinal orientation after axon injury.
机译：周围和中枢神经系统（CNS）损伤后功能的实质恢复严重取决于整个损伤部位的纵向轴突再生，这需要机械引导来提供支架。先前我们已经表明，具有定义的毛细管直径（25毫米）的各向异性藻酸盐基水凝胶是通过二价阳离子单向扩散到藻酸钠溶胶中的自组织过程形成的，促进了CNS轴突在体外和纵向的伸长。体内。在本研究中，在体外背根神经节向外生长试验中评估了各种毛细管直径和明胶掺入促进定向轴突生长和雪旺氏细胞迁移的影响。将藻酸盐溶胶与含Cu（2 +），Sr（2+）或Zn（2+）离子的溶液叠加，可以分别形成直径为18、25和55微米的毛细管的水凝胶。根据毛细血管结构内的轴突长度/密度和雪旺氏细胞密度来分析轴突生长和雪旺氏细胞迁移。随着毛细血管直径的增加，轴突向毛细管水凝胶的向内生长（始终伴随着雪旺氏细胞）得以增强。明胶的掺入不影响总轴突密度，但促进了水凝胶内轴突向外生长的长度。轴突的纵向取向在较宽的毛细管中下降，这表明中型毛细管是在轴突损伤后引起实质性轴突生长和纵向取向的最佳底物。

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