• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta biomaterialia >Folic acid-conjugated core/shell ZnS:Mn/ZnS quantum dots as targeted probes for two photon fluorescence imaging of cancer cells.
【24h】

Folic acid-conjugated core/shell ZnS:Mn/ZnS quantum dots as targeted probes for two photon fluorescence imaging of cancer cells.

机译：叶酸偶联的核/壳ZnS：Mn / ZnS量子点作为癌细胞的两个光子荧光成像的目标探针。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This work presents a novel approach to producing water soluble manganese-doped core/shell ZnS/ZnS quantum dots (ZnS:Mn/ZnS). The Mn-doped ZnS core was prepared through a nucleation doping strategy and a ZnS shell was grown on ZnS:Mn d-dots by decomposition of Zn(2+)-3-mercaptopropionic acid (MPA) complexes at 100 degrees C. It was found that the Mn2+(4)T1-->6A1 fluorescence emission at approximately 590 nm significantly increased after growth of the shell when the Mn2+ doping content was 4.0 at.%. A photoluminescence quantum yield of approximately 22% was obtained for core/shell nanocrystals. The nanoparticles were structurally and compositionally characterized by transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, and dynamic light scattering. The surface MPA molecules favor the dispersion of ZnS:Mn/ZnS QDs in aqueous media and make possible conjugation with targeting folic acid molecules. The folate receptor-mediated delivery of folic acid-conjugated ZnS:Mn/ZnS QDs was demonstrated using confocal microscopy with biphotonic excitation. Bare and folate-conjugated QDs exhibit only weak cytotoxicity towards folate receptor-positive T47D cancer cells and MCF-7 cells, used as a reference, at high concentrations (mmolar range) after 72h incubation.
机译：这项工作提出了一种生产水溶性锰掺杂核/壳ZnS / ZnS量子点（ZnS：Mn / ZnS）的新颖方法。通过成核掺杂策略制备了Mn掺杂的ZnS核，并通过在100摄氏度下分解Zn（2 +）-3-巯基丙酸（MPA）络合物在ZnS：Mn d点上生长ZnS壳。发现当Mn2 +掺杂含量为4.0 at。％时，在壳生长后，大约590 nm处的Mn2 +（4）T1-> 6A1荧光发射显着增加。对于核/壳纳米晶体，获得了约22％的光致发光量子产率。通过透射电子显微镜，X射线衍射，X射线光电子能谱和动态光散射对纳米颗粒进行结构和组成表征。表面MPA分子有利于ZnS：Mn / ZnS QD在水性介质中的分散，并可能与目标叶酸分子结合。叶酸受体介导的叶酸共轭的ZnS：Mn / ZnS QD的传递使用双光子激发共聚焦显微镜证实。温育72h后，裸露和叶酸结合的QD仅以高浓度（mmolar范围）对叶酸受体阳性的T47D癌细胞和MCF-7细胞（作为参考）表现出较弱的细胞毒性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号