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首页> 外文期刊>Acta biomaterialia >Effect of cleaning and sterilization on titanium implant surface properties and cellular response
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Effect of cleaning and sterilization on titanium implant surface properties and cellular response

机译：清洁和灭菌对钛植入物表面性能和细胞反应的影响

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Titanium (Ti) has been widely used as an implant material due to the excellent biocompatibility and corrosion resistance of its oxide surface. Biomaterials must be sterile before implantation, but the effects of sterilization on their surface properties have been less well studied. The effects of cleaning and sterilization on surface characteristics were bio-determined using contaminated and pure Ti substrata first manufactured to present two different surface structures: pretreated titanium (PT, Ra = 0.4 μm) (i.e. surfaces that were not modified by sandblasting and/or acid etching); (SLA, Ra = 3.4 μm). Previously cultured cells and associated extracellular matrix were removed from all bio-contaminated specimens by cleaning in a sonicator bath with a sequential acetone-isopropanol-ethanol- distilled water protocol. Cleaned specimens were sterilized with autoclave, gamma irradiation, oxygen plasma, or ultraviolet light. X-ray photoelectron spectroscopy (XPS), contact angle measurements, profilometry, and scanning electron microscopy were used to examine surface chemical components, hydrophilicity, roughness, and morphology, respectively. Small organic molecules present on contaminated Ti surfaces were removed with cleaning. XPS analysis confirmed that surface chemistry was altered by both cleaning and sterilization. Cleaning and sterilization affected hydrophobicity and roughness. These modified surface properties affected osteogenic differentiation of human MG63 osteoblast-like cells. Specifically, autoclaved SLA surfaces lost the characteristic increase in osteoblast differentiation seen on starting SLA surfaces, which was correlated with altered surface wettability and roughness. These data indicated that recleaned and resterilized Ti implant surfaces cannot be considered the same as the first surfaces in terms of surface properties and cell responses. Therefore, the reuse of Ti implants after resterilization may not result in the same tissue responses as found with never-before-implanted specimens.
机译：钛（Ti）由于其氧化物表面优异的生物相容性和耐腐蚀性而被广泛用作植入材料。生物材料在植入前必须是无菌的，但是灭菌对其表面性质的影响还没有得到很好的研究。清洁和灭菌对表面特性的影响是使用污染的纯Ti基质生物测定的，该基质首先被制造为具有两种不同的表面结构：预处理的钛（PT，Ra = 0.4μm）（即未经喷砂和/或未改性的表面）酸蚀）; （SLA，Ra ＝3.4μm）。通过在超声浴中用连续的丙酮-异丙醇-乙醇-蒸馏水操作规程清洗，从所有生物污染的标本中除去先前培养的细胞和相关的细胞外基质。用高压釜，伽马射线，氧等离子体或紫外线对清洁的样品进行灭菌。使用X射线光电子能谱（XPS），接触角测量，轮廓测定法和扫描电子显微镜分别检查表面化学成分，亲水性，粗糙度和形态。清洁除去存在于受污染的Ti表面上的有机小分子。 XPS分析证实，清洁和灭菌都会改变表面化学性质。清洁和灭菌会影响疏水性和粗糙度。这些修饰的表面性质影响了人MG63成骨细胞样细胞的成骨分化。具体而言，高压灭菌的SLA表面失去了在初始SLA表面上看到的成骨细胞分化的特征性增加，这与表面润湿性和粗糙度的变化相关。这些数据表明，就表面性质和细胞反应而言，重新清洁和再消毒的Ti植入物表面不能认为与第一表面相同。因此，再灭菌后再使用Ti植入物可能不会产生与从未植入过的标本相同的组织反应。

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