• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta biomaterialia >Preservation of FGF-2 bioactivity using heparin-based nanoparticles, and their delivery from electrospun chitosan fibers
【24h】

Preservation of FGF-2 bioactivity using heparin-based nanoparticles, and their delivery from electrospun chitosan fibers

机译：使用基于肝素的纳米颗粒保存FGF-2的生物活性，以及​​从静电纺丝的壳聚糖纤维中递送

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Here we present a novel matrix-mimetic nanoassembly based on polysaccharides. Chitosan electrospun fiber networks are decorated with heparin-containing polyelectrolyte complex nanoparticles (PCNs) that present basic fibroblast growth factor (FGF-2), both stably adsorbed to the surfaces and released into solution. These FGF-2/PCN complexes can be released from the fibers with zero-order kinetics over a period of 30 days. Further modification of fibers with a single bilayer of polyelectrolyte multilayer (PEM) composed of N,N,N-trimethyl chitosan and heparin completely prevent release, and the FGF-2/PCN complexes are retained on the fibers for the duration of the release experiment (30 days). We also compare the mitogenic activity of these FGF-2/PCN complexes delivered in two different states: adsorbed to a surface and dissolved in solution. FGF-2/PCN complexes exhibit mitogenic activity with respect to ovine bone marrow-derived mesenchymal stem cells, even after being preconditioned by incubating for 27 days at 37°C in solution. However, when the FGF-2/PCN complexes are adsorbed to chitosan and coated with PEMs, the mitogenic activity of the FGF-2 steadily decreases with increasing preconditioning time. This work demonstrates a new system for stabilizing and controlling the delivery of heparin-binding growth factors, using polysaccharide-based matrix-mimetic nanomaterials. This work also contributes to our understanding of the preferred mode of growth factor delivery from porous scaffolds.
机译：在这里，我们提出了一种基于多糖的新型基质模拟纳米组装。壳聚糖电纺纤维网络用含肝素的聚电解质复合物纳米颗粒（PCN）修饰，该颗粒具有基本的成纤维细胞生长因子（FGF-2），既能稳定地吸附在表面上又释放到溶液中。这些FGF-2 / PCN复合物可以在30天内以零级动力学从纤维中释放出来。用由N，N，N-三甲基壳聚糖和肝素组成的单双层聚电解质多层（PEM）进一步修饰纤维可完全防止释放，并且在释放实验期间FGF-2 / PCN复合物会保留在纤维上（30天）。我们还比较了以两种不同状态递送的这些FGF-2 / PCN复合物的促有丝分裂活性：吸附到表面并溶解在溶液中。甚至在通过在37°C的溶液中孵育27天进行预处理后，FGF-2 / PCN复合物对绵羊骨髓来源的间充质干细胞也显示出有丝分裂活性。但是，当FGF-2 / PCN复合物吸附到壳聚糖上并涂有PEM时，随着预处理时间的增加，FGF-2的促有丝分裂活性逐渐降低。这项工作演示了使用基于多糖的基质模拟纳米材料稳定和控制肝素结合生长因子传递的新系统。这项工作也有助于我们了解从多孔支架中输送生长因子的首选方式。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号