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首页> 外文期刊>Acta biomaterialia >Oriented lamellar silk fibrous scaffolds to drive cartilage matrix orientation: Towards annulus fibrosus tissue engineering
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Oriented lamellar silk fibrous scaffolds to drive cartilage matrix orientation: Towards annulus fibrosus tissue engineering

机译：定向的层状丝状纤维支架驱动软骨基质的定向：朝向纤维环组织工程

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摘要

A novel design of silk-based scaffold is developed using a custom-made winding machine, with fiber alignment resembling the anatomical criss-cross lamellar fibrous orientation features of the annulus fibrosus of the intervertebral disc. Crosslinking of silk fibroin fibers with chondroitin sulphate (CS) was introduced to impart superior biological functionality. The scaffolds, with or without CS, instructed alignment of expanded human chondrocytes and of the deposited extracellular matrix while supporting their chondrogenic redifferentiation. The presence of CS crosslinking could not induce statistically significant changes in the measured collagen or glycosaminoglycan content, but resulted in an increased construct stiffness. By offering the combined effect of cell/matrix alignment and chondrogenic support, the silk fibroin scaffolds developed with precise fiber orientation in lamellar form represent a suitable substrate for tissue engineering of the annulus fibrosus part of the intervertebral disc.
机译：使用定制绕线机开发了一种新颖的基于丝绸的支架设计，其纤维排列类似于椎间盘环纤维的解剖纵横交错的层状纤维取向特征。引入了丝素蛋白纤维与硫酸软骨素（CS）的交联以赋予卓越的生物学功能。具有或不具有CS的支架指示扩增的人软骨细胞和沉积的细胞外基质的排列，同时支持它们的软骨原性再分化。 CS交联的存在不能在测量的胶原蛋白或糖胺聚糖含量上引起统计学上的显着变化，但会导致构建物刚度增加。通过提供细胞/基质排列和软骨形成支持的综合作用，以层状形式以精确的纤维取向开发的丝素蛋白支架代表了椎间盘纤维环部分组织工程化的合适基质。

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