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首页> 外文期刊>Acta biomaterialia >Combining self-assembling peptide gels with three-dimensional elastomer scaffolds
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Combining self-assembling peptide gels with three-dimensional elastomer scaffolds

机译：将自组装肽凝胶与三维弹性体支架相结合

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摘要

Some of the problems raised by the combination of porous scaffolds and self-assembling peptide (SAP) gels as constructs for tissue engineering applications are addressed for the first time. Scaffolds of poly(ethyl acrylate) and the SAP gel RAD16-I were employed. The in situ gelation of the SAP gel inside the pores of the scaffolds was studied. The scaffold-cum-gel constructs were characterized morphologically, physicochemically and mechanically. The possibility of incorporating an active molecule (bovine serum albumin, taken here as a model molecule for others) in the gel within the scaffold's pores was assessed, and the kinetics of its release in phosphate-buffered saline was followed. Cell seeding and colonization of these constructs were preliminarily studied with L929 fibroblasts and subsequently checked with sheep adipose-tissue-derived stem cells intended for further preclinical studies. Static (conventional) and dynamically assisted seedings were compared for bare scaffolds and the scaffold-cum-gel constructs. The SAP gel inside the pores of the scaffold significantly improved the uniformity and density of cell colonization of the three-dimensional (3-D) structure. These constructs could be of use in different advanced tissue engineering applications, where, apart from a cell-friendly extracellular matrix -like aqueous environment, a larger-scale 3-D structure able to keep the cells in a specific place, give mechanical support and/or conduct spatially the tissue growth could be required.
机译：首次解决了多孔支架和自组装肽（SAP）凝胶作为组织工程应用构建体的组合所引起的一些问题。使用了聚丙烯酸乙酯的支架和SAP凝胶RAD16-I。研究了支架孔内部SAP凝胶的原位凝胶化。支架-凝胶构建体的形态，物理化学和机械特征被表征。评估了将活性分子（牛血清白蛋白，在这里用作其他分子的模型分子）掺入到支架孔内的凝胶中的可能性，并跟踪了其在磷酸盐缓冲液中的释放动力学。使用L929成纤维细胞初步研究了这些构建体的细胞播种和定植，随后使用绵羊脂肪组织来源的干细胞进行了检查，以用于进一步的临床前研究。比较了裸棚架和棚架兼凝胶构建物的静态（常规）播种和动态辅助播种。支架孔内部的SAP凝胶显着提高了三维（3-D）结构细胞定植的均匀性和密度。这些构建体可用于不同的高级组织工程应用中，在这些应用中，除了像细胞友好的细胞外基质之类的水性环境外，更大规模的3-D结构能够将细胞保持在特定位置，并提供机械支撑和/或在空间上进行可能需要组织生长。

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