• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta biomaterialia >Combining decellularized human adipose tissue extracellular matrix and adipose-derived stem cells for adipose tissue engineering
【24h】

Combining decellularized human adipose tissue extracellular matrix and adipose-derived stem cells for adipose tissue engineering

机译：将脱细胞的人脂肪组织细胞外基质与脂肪来源的干细胞结合起来，用于脂肪组织工程

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Repair of soft tissue defects resulting from lumpectomy or mastectomy has become an important rehabilitation process for breast cancer patients. This study aimed to provide an adipose tissue engineering platform for soft tissue defect repair by combining decellularized human adipose tissue extracellular matrix (hDAM) and human adipose-derived stem cells (hASCs). To derive hDAM incised human adipose tissues underwent a decellularization process. Effective cell removal and lipid removal were proved by immunohistochemical analysis and DNA quantification. Scanning electron microscopic examination showed a three-dimensional nanofibrous architecture in hDAM. The hDAM included collagen, sulfated glycosaminoglycan, and vascular endothelial growth factor, but lacked major histocompatibility complex antigen I. hASC viability and proliferation on hDAM were proven in vitro. hDAM implanted subcutaneously in Fischer rats did not cause an immunogenic response, and it underwent remodeling, as indicated by host cell infiltration, neovascularization, and adipose tissue formation. Fresh fat grafts (Coleman technique) and engineered fat grafts (hDAM combined with hASCs) were implanted subcutaneously in nude rats. The implanted engineered fat grafts maintained their volume for 8 weeks, and the hASCs contributed to adipose tissue formation. In summary, the combination of hDAM and hASCs provides not only a clinically translatable platform for adipose tissue engineering, but also a vehicle for elucidating fat grafting mechanisms.
机译：乳房切除术或乳房切除术导致的软组织缺损的修复已成为乳腺癌患者的重要康复过程。这项研究旨在通过结合脱细胞的人脂肪组织细胞外基质（hDAM）和人脂肪来源的干细胞（hASCs），为软组织缺损修复提供一个脂肪组织工程平台。为了获得hDAM，将切开的人脂肪组织进行了脱细胞过程。通过免疫组织化学分析和DNA定量证明有效的细胞去除和脂质去除。扫描电子显微镜检查显示了hDAM中的三维纳米纤维结构。 hDAM包括胶原蛋白，硫酸化的糖胺聚糖和血管内皮生长因子，但缺乏主要的组织相容性复合抗原I。hASC在hDAM上的生存力和增殖能力得到了证实。如宿主细胞浸润，新血管形成和脂肪组织形成所示，在Fischer大鼠中皮下植入的hDAM不会引起免疫原性应答，并且进行了重塑。将新鲜的脂肪移植物（科尔曼技术）和工程化的脂肪移植物（hDAM与hASC结合）皮下植入裸鼠。植入的工程化脂肪移植物维持其体积8周，而hASC促进了脂肪组织的形成。总之，hDAM和hASC的结合不仅为脂肪组织工程提供了可临床翻译的平台，而且为阐明脂肪移植机制提供了载体。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号