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首页> 外文期刊>Acta biomaterialia >Osteogenic and angiogenic potentials of monocultured and co-cultured human-bone-marrow-derived mesenchymal stem cells and human-umbilical-vein endothelial cells on three-dimensional porous beta-tricalcium phosphate scaffold
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Osteogenic and angiogenic potentials of monocultured and co-cultured human-bone-marrow-derived mesenchymal stem cells and human-umbilical-vein endothelial cells on three-dimensional porous beta-tricalcium phosphate scaffold

机译：在三维多孔β-磷酸三钙支架上单培养和共培养人骨髓间充质干细胞和人脐静脉内皮细胞的成骨和血管生成潜力

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The use of biodegradable beta-tricalcium phosphate (β-TCP) scaffolds holds great promise for bone tissue engineering. However, the effects of β-TCP on bone and endothelial cells are not fully understood. This study aimed to investigate cell proliferation and differentiation of mono- or co-cultured human-bone-marrow-derived mesenchymal stem cells (hBMSCs) and human-umbilical-vein endothelial cells (HUVECs) on a three-dimensional porous, biodegradable β-TCP scaffold. In co-culture studies, the ratios of hBMSCs:HUVECs were 5:1, 1:1 and 1:5. Cellular morphologies of HUVECs, hBMSCs and co-cultured HUVECs/hBMSCs on the β-TCP scaffolds were monitored using confocal and scanning electron microscopy. Cell proliferation was monitored by measuring the amount of double-stranded DNA (dsDNA) whereas hBMSC and HUVEC differentiation was assessed using the osteogenic and angiogenic markers, alkaline phosphatase (ALP) and PECAM-1 (CD31), respectively. Results show that HUVECs, hBMSCs and hBMSCs/HUVECs adhered to and proliferated well on the β-TCP scaffolds. In monoculture, hBMSCs grew faster than HUVECs on the β-TCP scaffolds after 7 days, but HUVECs reached similar levels of proliferation after 14 days. In monoculture, β-TCP scaffolds promoted ALP activities of both hBMSCs and HUVECs when compared to those grown on tissue culture well plates. ALP activity of cells in co-culture was higher than that of hBMSCs in monoculture. Real-time polymerase chain reaction results indicate that runx2 and alp gene expression in monocultured hBMSCs remained unchanged at days 7 and 14, but alp gene expression was significantly increased in hBMSC co-cultures when the contribution of individual cell types was not distinguished.
机译：使用可生物降解的β-磷酸三钙（β-TCP）支架对骨组织工程具有广阔的前景。然而，β-TCP对骨和内皮细胞的作用尚不完全清楚。这项研究旨在研究在三维多孔，可生物降解的β-细胞上单或共培养的人骨髓间充质干细胞（hBMSC）和人脐静脉内皮细胞（HUVEC）的细胞增殖和分化。 TCP支架。在共培养研究中，hBMSC：HUVEC的比例为5：1、1：1和1：5。使用共聚焦和扫描电子显微镜监测在β-TCP支架上的HUVEC，hBMSC和共培养的HUVEC / hBMSC的细胞形态。通过测量双链DNA（dsDNA）的量来监测细胞增殖，而分别使用成骨和血管生成标记碱性磷酸酶（ALP）和PECAM-1（CD31）评估hBMSC和HUVEC的分化。结果显示，HUVEC，hBMSC和hBMSC / HUVEC在β-TCP支架上粘附并良好增殖。在单培养中，在7天后，hBMSC在β-TCP支架上的生长速度快于HUVEC，但在14天后，HUVEC达到了相似的增殖水平。在单培养中，与在组织培养孔板上生长的支架相比，β-TCP支架可促进hBMSC和HUVEC的ALP活性。共培养细胞的ALP活性高于单培养hBMSCs。实时聚合酶链反应结果表明，在单一培养的hBMSC中，runx2和alp基因表达在第7天和第14天保持不变，但是在hBMSC共培养中，当不区分单个细胞类型的贡献时，alp基因表达显着增加。

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