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首页> 外文期刊>Acta biomaterialia >Human mesenchymal stem cell culture on heparin-based hydrogels and the modulation of interactions by gel elasticity and heparin amount.
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Human mesenchymal stem cell culture on heparin-based hydrogels and the modulation of interactions by gel elasticity and heparin amount.

机译：在基于肝素的水凝胶上培养人间充质干细胞，并通过凝胶弹性和肝素量调节相互作用。

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Human adipose-derived stem cells (hADSCs) are a promising cell source for tissue engineering and regenerative medicine with no ethnical issue and easy access of large quantities. Conventional surfaces for hADSC culture, such as tissue culture plates (TCPs), do not provide optimal environmental cues, leading to limited expansion, loss of pluripotency and undesirable differentiation of stem cells. The present study demonstrated that heparin-based hydrogels without additional modification provided an excellent surface for adhesion and proliferation of hADSCs, which were further tunable by both the amount of heparin (in a positive way) and the elasticity of hydrogel (in a negative way). The optimized heparin-based hydrogel could selectively modulate the adhesion of hADSCs and human bone marrow stem cells (but not all kinds of cells), and resulted in a significant increase in cell proliferation compared to TCP. Furthermore, in terms of the maintenance of pluripotency and specific differentiation, heparin-based hydrogel was much superior to TCP. The selective binding and proliferation of human mesenchymal stem cells on heparin-based hydrogel over other hydrogels were largely mediated by integrin β1 and selectin, and these superior characteristics were observed regardless of the presence of serum proteins in the culture medium. Consequently, heparin-based hydrogel could be a powerful platform for cultivation of mesenchymal stem cells in various applications.
机译：人脂肪来源的干细胞（hADSCs）是组织工程和再生医学的有希望的细胞来源，没有种族问题，并且易于大量获取。用于hADSC培养的常规表面（例如组织培养板（TCP））无法提供最佳的环境线索，从而导致有限的扩增，多能性损失和干细胞的不良分化。本研究表明，未经其他修饰的基于肝素的水凝胶为hADSCs的粘附和增殖提供了出色的表面，而肝素的量（以积极的方式）和水凝胶的弹性（以消极的方式）均可进一步调节这些表面。优化的基于肝素的水凝胶可以选择性地调节hADSCs和人骨髓干细胞（但不是所有类型的细胞）的粘附，与TCP相比，可显着增加细胞增殖。此外，就维持多能性和特异性分化而言，基于肝素的水凝胶远远优于TCP。人间充质干细胞在基于肝素的水凝胶上相对于其他水凝胶的选择性结合和增殖在很大程度上由整联蛋白β1和选择素介导，并且无论培养基中是否存在血清蛋白，都观察到了这些优越的特性。因此，基于肝素的水凝胶可以成为在各种应用中培养间充质干细胞的强大平台。

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