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首页> 外文期刊>Acta biomaterialia >Enhanced differentiation of human neural crest stem cells towards the Schwann cell lineage by aligned electrospun fiber matrix.
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Enhanced differentiation of human neural crest stem cells towards the Schwann cell lineage by aligned electrospun fiber matrix.

机译：通过对齐的电纺纤维基质增强了人类神经c干细胞向雪旺氏细胞谱系的分化。

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摘要

Human pluripotent stem cell-derived neural crest stem cells (NCSCs) provide a promising cell source for generating Schwann cells in the treatment of neurodegenerative diseases and traumatic injuries in the peripheral nervous system. Influencing cell behavior through a synthetic matrix topography has been shown to be an effective approach to directing stem cell proliferation and differentiation. Here we have investigated the effect of nanofiber topography on the differentiation of human embryonic stem cell-derived NCSCs towards the Schwann cell lineage. Using electrospun fibers of different diameters and alignments we demonstrated that aligned fiber matrices effectively induced cell alignment, and that fiber matrices with average diameters of 600nm and 1.6μm most effectively promoted NCSC differentiation towards the Schwann cell lineage compared with random fibers and two-dimensional tissue culture plates. More importantly, human NCSCs that were predifferentiated in Schwann cell medium for 2weeks exhibited higher sensitivity to the aligned fiber topography than undifferentiated NCSCs. This study provides an efficient protocol for Schwann cell derivation by combining an aligned nanofiber matrix and an optimized differentiation medium, and highlights the importance of matching extrinsic matrix signaling with cell intrinsic programming in a temporally specific manner.
机译：人多能干细胞衍生的神经rest干细胞（NCSC）为在治疗神经退行性疾病和周围神经系统的创伤性损伤中产生雪旺细胞提供了有希望的细胞来源。通过合成基质形貌影响细胞行为已被证明是指导干细胞增殖和分化的有效方法。在这里，我们研究了纳米纤维形貌对人类胚胎干细胞来源的NCSC向雪旺氏细胞谱系分化的影响。使用不同直径和排列的电纺纤维，我们证明了排列的纤维基质可以有效地诱导细胞排列，与随机纤维和二维组织相比，平均直径为600nm和1.6μm的纤维基质最有效地促进了NCSC向雪旺氏细胞谱系的分化。培养板。更重要的是，在雪旺氏细胞培养基中预分化2周的人NCSC与未分化的NCSC相比，对对齐的纤维形貌显示出更高的敏感性。这项研究通过结合对齐的纳米纤维基质和优化的分化培养基，为雪旺细胞的衍生提供了有效的协议，并强调了以时间特定方式将外在基质信号与细胞内在编程相匹配的重要性。

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