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首页> 外文期刊>Acta biomaterialia >Preparation and characterization of a novel tobramycin-containing antibacterial collagen film for corneal tissue engineering
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Preparation and characterization of a novel tobramycin-containing antibacterial collagen film for corneal tissue engineering

机译：用于角膜组织工程的新型含妥布霉素的抗菌胶原膜的制备与表征

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Corneal disease is a major cause of blindness and keratoplasty is an effective treatment method. However, clinical treatment is limited due to a severe shortage of high-quality allogeneic corneal tissues and the bacterial infection after corneal transplantation. In this study, we develop a novel artificial and antibacterial collagen film (called Col-Tob) for corneal repair. In the Col-Tob film, the tobramycin, which is an aminoglycoside antibiotic to treat various types of bacterial infections, was cross-linked by 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide and N-hydroxysuccinimide onto the collagen. Physical properties, antibacterial property and biocompatibility of the films were characterized. The results indicate that the film is basically transparent and has appropriate mechanical properties. Cell experiments show that human corneal epithelial cells could adhere to and proliferate well on the film. Most importantly, the film exhibits excellent antibacterial effect in vitro. Lamellar keratoplasty shows that the Col-Tob film can be sutured in rabbit eyes and are epithelialized completely in15 ± 5 days, and their transparency is restored quickly in the first month. Corneal rejection reaction, neovascularization and keratoconus are not observed within 3 months. This film, which can be prepared in large quantities and at low cost, should have potential application in corneal repair.
机译：角膜疾病是失明的主要原因，角膜移植术是一种有效的治疗方法。然而，由于高质量异体角膜组织的严重短缺和角膜移植后的细菌感染，临床治疗受到限制。在这项研究中，我们开发了一种新型的人工和抗菌胶原膜（称为Col-Tob），用于角膜修复。在Col-Tob膜中，妥布霉素是一种用于治疗各种细菌感染的氨基糖苷类抗生素，它通过1-乙基-3-（3-二甲基氨基丙基）碳二亚胺和N-羟基琥珀酰亚胺交联到胶原上。表征了膜的物理性质，抗菌性质和生物相容性。结果表明该膜基本上是透明的并且具有适当的机械性能。细胞实验表明，人角膜上皮细胞可以粘附并很好地在膜上增殖。最重要的是，该膜在体外表现出优异的抗菌作用。层状角膜移植术表明，可以在兔眼中缝合Col-Tob膜，并在15±5天内完全上皮化，并且在第一个月内其透明度很快恢复。在3个月内未观察到角膜排斥反应，新血管形成和圆锥角膜。这种可以大量低成本生产的薄膜在角膜修复中应具有潜在的应用前景。

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