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首页> 外文期刊>Acta Dermato-Venereologica >Expression of p53 protein before and after PUVA treatment in psoriasis.
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Expression of p53 protein before and after PUVA treatment in psoriasis.

机译：银屑病PUVA治疗前后p53蛋白的表达。

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We investigated the effect of the potentially carcinogenic psoralen plus UVA radiation (PUVA) therapy on the expression of p53 in skin of psoriatic patients. p53 antibodies DO7 and Pab240, antibodies against PCNA and Ki67 and the avidin-biotin immunoperoxidase complex method were used in the immunohistochemical staining of biopsy samples from non-lesional and lesional skin of 23 patients who received either trioxsalen bath PUVA or oral 8-methoxypsoralen PUVA. Biopsies were taken before and after a PUVA course. A modest expression of p53 was seen in psoriatic lesions in 17/21 patients before any treatment, probably as a physiological reaction to the hyperproliferation. Both p53 and the proliferation markers Ki67 and PCNA followed the same pattern, being more frequent in psoriatic lesions than in non-lesional skin. Exposure to PUVA induced an increase in p53 expression in non-lesional skin in 14/19 patients, putatively as a response to DNA damage caused by PUVA. In psoriatic lesions about half of the patients showed increased and half decreased expression of p53. The latter finding might be explained by decreased proliferation activity of the healing epidermis. In conclusion, p53 nuclear positivity in non-lesional skin after PUVA treatment is likely to be induced by DNA damage caused by PUVA, while in psoriatic lesions it could be a result of the combined effect of decreasing epidermal proliferation and DNA-damage.
机译：我们调查了潜在致癌性补骨脂素加UVA辐射（PUVA）治疗对银屑病患者皮肤中p53表达的影响。 p53抗体DO7和Pab240，抗PCNA和Ki67的抗体以及抗生物素蛋白-生物素免疫过氧化物酶复合物方法用于23例接受三氧沙仑浴PUVA或口服8-甲氧基补骨脂素PUVA的患者的非病变和病变皮肤的活检样品的免疫组织化学染色。在PUVA疗程之前和之后进行活检。在进行任何治疗之前，17/21患者的银屑病皮损中可见p53适度表达，这可能是对过度增殖的生理反应。 p53和增殖标志物Ki67和PC​​NA都遵循相同的模式，在银屑病皮损中比在非皮损皮肤中更常见。暴露于PUVA导致14/19患者非病变皮肤中p53表达增加，推测是对PUVA引起的DNA损伤的反应。在银屑病皮损中，大约一半的患者显示p53表达增加而一半降低。后一个发现可能是由于愈合表皮的增殖活性降低所致。总之，PUVA处理后非病变皮肤的p53核阳性很可能是由PUVA引起的DNA损伤诱导的，而在牛皮癣病变中，这可能是减少表皮增殖和DNA损伤共同作用的结果。

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