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首页> 外文期刊>Acta biomaterialia >A novel three-dimensional aerogel biochip for molecular recognition of nucleotide acids.
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A novel three-dimensional aerogel biochip for molecular recognition of nucleotide acids.

机译：一种新型的三维气凝胶生物芯片，用于核苷酸分子的分子识别。

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Mesoporous aerogel was produced under regular atmospheric conditions using the sol-gel polymerization of tetraethyl orthosilicate with an ionic liquid as both solvent and active agent. This was then used to build a three-dimensional structure to recognize nucleotide acids. Fourier transformation infrared spectroscopy, scanning electron microscopy, (29)Si solid-state nuclear magnetic resonance, and Brunauer-Emmett-Teller instruments were used to characterize this 3D aerogel, demonstrating that it had high porosity and large internal networking surface area that could capture nucleotide acids. The functionality of molecular recognition on nucleotide acids was demonstrated by immobilizing an oligonucleotide to probe its DNA target and confirming the tagged fluorescent signals by confocal laser scanning microscopy. The results indicated that the as-prepared 3D bioaerogel was capable of providing a very large surface area to capture and recognize human gene ATP5O.
机译：使用原硅酸四乙酯与离子液体作为溶剂和活性剂的溶胶-凝胶聚合，在常规的大气条件下生产中孔气凝胶。然后将其用于构建三维结构以识别核苷酸。使用傅里叶变换红外光谱法，扫描电子显微镜，（29）Si固态核磁共振和Brunauer-Emmett-Teller仪器对这种3D气凝胶进行表征，表明其具有高孔隙率和可捕获的较大内部网络表面积核苷酸酸。通过固定寡核苷酸以探测其DNA靶标并通过共聚焦激光扫描显微镜确认标记的荧光信号，证明了在核苷酸酸上进行分子识别的功能。结果表明，所制备的3D生物气凝胶能够提供非常大的表面积来捕获和识别人类基因ATP5O。

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