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首页> 外文期刊>Acta biomaterialia >Sustained plasmid DNA release from dissolving mineral coatings.
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Sustained plasmid DNA release from dissolving mineral coatings.

机译：从溶解的矿物涂层中持续释放质粒DNA。

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摘要

Calcium phosphate (CaP) minerals such as hydroxyapatite are able to bind a diverse range of biological molecules due to the presence of anions and cations in their crystal structure. The well-characterized ability of CaP minerals to bind and release plasmid DNA, coupled with the ability of biodegradable CaP coatings to form on the surface of common biomaterials, provides a potential mechanism for controlled release of plasmid DNA from various biomaterials. In this study we hypothesized that the release of plasmid DNA from CaP coatings formed on poly(lactide-co-glycolide) (PLG) substrates would be dependent on both the intrinsic properties of the CaP mineral coating and the surrounding solution conditions. Experiments were designed to consider two general parameters: (i) the stability of various CaP mineral coatings in solution environments that are relevant to physiological conditions; (ii) the relationship between mineral stability and sustained plasmid DNA release. Our results corroborate previous studies that have demonstrated a direct relationship between intrinsic mineral composition and mineral stability. In addition, we further demonstrate that ion composition and pH of the surrounding solution environment can significantly influence mineral stability. In turn, mineral stability significantly influenced release of plasmid DNA from mineral coatings in vitro, and the DNA release efficiency could be tuned by controlling the mineral properties in various solution environments. These CaP mineral coatings may be a useful platform for plasmid DNA delivery applications using various biomaterial platforms.
机译：磷酸钙（CaP）矿物（例如羟基磷灰石）由于其晶体结构中存在阴离子和阳离子，因此能够结合多种生物分子。 CaP矿物具有良好的结合和释放质粒DNA的能力，再加上可生物降解的CaP涂层在常见生物材料表面形成的能力，为从各种生物材料中控制释放质粒DNA提供了潜在的机制。在这项研究中，我们假设质粒DNA从在聚（丙交酯-共-乙交酯）（PLG）底物上形成的CaP涂层的释放将取决于CaP矿物涂层的内在特性和周围溶液的条件。设计实验时要考虑两个通用参数：（i）各种CaP矿物涂层在与生理条件有关的溶液环境中的稳定性; （ii）矿物稳定性与质粒DNA持续释放之间的关系。我们的结果证实了先前的研究，这些研究表明固有矿物成分与矿物稳定性之间存在直接关系。此外，我们进一步证明了周围溶液环境的离子组成和pH值可以显着影响矿物的稳定性。反过来，矿物质的稳定性显着影响了质粒DNA在体外从矿物质涂层中的释放，并且可以通过控制各种溶液环境中的矿物质性质来调节DNA的释放效率。这些CaP矿物涂层可能是使用各种生物材料平台进行质粒DNA递送应用的有用平台。

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