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首页> 外文期刊>Acta biomaterialia >Immobilization of heparin on polysulfone surface for selective adsorption of low-density lipoprotein (LDL).
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Immobilization of heparin on polysulfone surface for selective adsorption of low-density lipoprotein (LDL).

机译：肝素固定在聚砜表面上以选择性吸附低密度脂蛋白（LDL）。

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摘要

A versatile method was developed to immobilize heparin covalently on polysulfone sheets (PSu) to achieve selective adsorption of low-density lipoprotein (LDL). This was achieved by activation of PSu with successive treatments of chlorodimethyl ether and ethylenediamine, and subsequent chemical binding of heparin with bifunctional linker molecules. A heparin density up to 0.86 microg cm(-2) on a dense PSu film was achieved. The modified PSu films were characterized by attenuated total reflectance Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The hydrophilicity of the PSu film was improved greatly by covalent immobilization of heparin. The water contact angle of PSu film was decreased from 86.6 + or - 3.7 degrees to 50.5 + or - 3.2 degrees after binding of 0.36 microg cm(-2) heparin. An enzyme-linked immunosorbent assay was used to measure the binding of LDL on plain and modified PSu films. It was found that the heparin-modified PSu film could selectively recognize LDL from binary protein solutions. Furthermore, it was possible to desorb LDL from heparinized PSu, but not from plain PSu, with heparin, sodium chloride or urea solution, which indicates a selective but reversible binding of LDL to heparin. The results suggest that heparin-modified PSu membranes are promising for application in simultaneous hemodialysis and LDL apheresis therapy.
机译：开发了一种通用的方法将肝素共价固定在聚砜片（PSu）上，以实现低密度脂蛋白（LDL）的选择性吸附。这是通过依次处理氯二甲醚和乙二胺激活PSu，然后将肝素与双功能连接分子化学结合而实现的。在致密的PSu膜上实现了高达0.86 microg cm（-2）的肝素密度。通过衰减全反射傅里叶变换红外光谱和X射线光电子能谱对改性的PSu膜进行表征。通过共价固定肝素，大大改善了PSu膜的亲水性。结合0.36 microg cm（-2）肝素后，PSu膜的水接触角从86.6 +或-3.7度降低到50.5 +或-3.2度。酶联免疫吸附试验用于测量LDL在普通和改性PSu膜上的结合。已经发现，肝素修饰的PSu膜可以选择性地从二元蛋白溶液中识别LDL。此外，可以用肝素，氯化钠或尿素溶液从肝素化的PSu中解吸LDL，但不能从普通PSu中解吸LDL，这表明LDL与肝素有选择性但可逆的结合。结果表明，肝素修饰的PSu膜有望用于同时进行的血液透析和LDL血液分离术。

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