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首页> 外文期刊>Acta biomaterialia >Development and characterization of rhVEGF-loaded poly(HEMA-MOEP) coatings electrosynthesized on titanium to enhance bone mineralization and angiogenesis.
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Development and characterization of rhVEGF-loaded poly(HEMA-MOEP) coatings electrosynthesized on titanium to enhance bone mineralization and angiogenesis.

机译：在钛上电合成rhVEGF的聚（HEMA-MOEP）涂层的开发和表征，以增强骨矿化和血管生成。

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摘要

Osteointegration of titanium implants could be significantly improved by coatings capable of promoting both mineralization and angiogenesis. In the present study, a copolymeric hydrogel coating, poly-2-hydroxyethyl methacrylate-2-methacryloyloxyethyl phosphate (P(HEMA-MOEP)), devised to enhance calcification in body fluids and to entrap and release growth factors, was electrosynthesized for the first time on titanium substrates and compared to poly-2-hydroxyethyl methacrylate (PHEMA), used as a blank reference. Polymers exhibiting negatively charged groups, such as P(HEMA-MOEP), help to enhance implant calcification. The electrosynthesized coatings were characterized by X-ray photoelectron spectroscopy and atomic force microscopy. MG-63 human osteoblast-like cell behaviour on the coated specimens was investigated by scanning electron microscopy, MTT viability test and osteocalcin mRNA detection. The ability of negatively charged phosphate groups to promote hydroxyapatite-like calcium phosphate deposition on the implants was explored by immersing them in simulated body fluid. Similar biological responses were observed in both coated specimens, while calcium-phosphorus globules were detected only on P(HEMA-MOEP) surfaces pretreated with alkaline solution. Testing of the ability of P(HEMA-MOEP) hydrogels to entrap and release human recombinant vascular endothelial growth factor, to tackle the problem of insufficient oxygen and nutrient delivery, suggested that P(HEMA-MOEP)-coated titanium prostheses could represent a multifunctional material suitable for bone restoration applications.
机译：能够促进矿化和血管生成的涂层可以显着改善钛植入物的骨整合。在本研究中，首次电合成了一种共聚水凝胶涂层，即聚甲基丙烯酸2-羟乙酯-甲基丙烯酸-2-甲基丙烯酰氧基乙基磷酸酯（P（HEMA-MOEP）），该涂层旨在增强体液中的钙化并捕获和释放生长因子。在钛基材上的时间，并与用作空白参考的聚甲基丙烯酸-2-羟乙酯（PHEMA）进行了比较。表现出负电荷基团的聚合物，例如P（HEMA-MOEP），有助于增强植入物的钙化。电合成涂层通过X射线光电子能谱和原子力显微镜表征。通过扫描电子显微镜，MTT生存力测试和骨钙素mRNA检测研究了MG-63人在涂层标本上的成骨样细胞行为。通过将带负电的磷酸基团浸入模拟体液中来研究其在植入物中促进羟基磷灰石样磷酸钙沉积的能力。在两个涂层样品中均观察到了相似的生物学反应，而钙磷小球仅在用碱性溶液预处理的P（HEMA-MOEP）表面上被检测到。测试P（HEMA-MOEP）水凝胶捕获和释放人重组血管内皮生长因子的能力，以解决氧气和养分输送不足的问题，表明P（HEMA-MOEP）涂层的钛假体可以代表一种多功能适用于骨骼修复应用的材料。

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