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首页> 外文期刊>Acta biomaterialia >Cyclically stretching developing tissue in vivo enhances mechanical strength and organization of vascular grafts.
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Cyclically stretching developing tissue in vivo enhances mechanical strength and organization of vascular grafts.

机译：在体内循环拉伸发育中的组织可增强机械强度和血管移植物的组织。

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Tissue-engineered vascular grafts must have qualities that rival native vasculature, specifically the ability to remodel, the expression of functional endothelial components and a dynamic and functional extracellular matrix (ECM) that resists the forces of the arterial circulation. We have developed a device that when inserted into the peritoneal cavity, attracts cells around a tubular scaffold to generate autologous arterial grafts. The device is capable of cyclically stretching (by means of a pulsatile pump) developing tissue to increase the mechanical strength of the graft. Pulsed (n=8) and unpulsed (n=8) devices were implanted for 10 days in Lovenaar sheep (n=8). Pulsation occurred for a period of 5-8 days before harvest. Thick unadhered autologous tissue with cells residing in a collagen ECM was produced in all devices. Collagen organization was greater in the circumferential direction of pulsed tissue. Immunohistochemical labelling revealed the hematopoietic origin of >90% cells and a significantly higher coexpression with vimentin in pulsed tissue. F-actin expression, mechanical failure strength and strain were also significantly increased by pulsation. Moreover, tissue could be grafted as carotid artery patches. This paper shows that unadhered tissue tubes with increased mechanical strength and differentiation in response to pulsation can be produced with every implant after a period of 10 days. However, these tissue tubes require a more fine-tuned exposure to pulsation to be suitable for use as vascular grafts.
机译：组织工程化的血管移植物必须具有与天然脉管系统相匹敌的品质，特别是其重构能力，功能性内皮成分的表达以及抵抗动脉循环力的动态和功能性细胞外基质（ECM）。我们已经开发出一种设备，当该设备插入腹膜腔时，可以吸引管状支架周围的细胞以生成自体动脉移植物。该装置能够周期性地（通过脉动泵）拉伸组织组织，以增加移植物的机械强度。在Lovenaar绵羊（n = 8）中植入脉冲（n = 8）和非脉冲（n = 8）装置10天。收获前脉动发生了5-8天。在所有装置中均产生了厚厚的未粘附的自体组织，其细胞位于胶原ECM中。胶原组织在脉冲组织的圆周方向上更大。免疫组织化学标记显示，> 90％细胞具有造血起源，并且在脉冲组织中与波形蛋白的共表达显着更高。 F-肌动蛋白的表达，机械破坏强度和应变也通过脉动显着增加。此外，可以将组织移植为颈动脉斑块。本文显示，经过10天的植入后，每个植入物都可以生产出机械强度高，响应搏动而分化的未粘附组织管。然而，这些组织管需要更微调的脉动暴露以适合用作血管​​移植物。

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