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首页> 外文期刊>Acta biomaterialia >An in vitro study of two GAG-like marine polysaccharides incorporated into injectable hydrogels for bone and cartilage tissue engineering.
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An in vitro study of two GAG-like marine polysaccharides incorporated into injectable hydrogels for bone and cartilage tissue engineering.

机译：两种GAG样海洋多糖掺入可注射水凝胶的骨和软骨组织工程的体外研究。

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Natural polysaccharides are attractive compounds with which to build scaffolds for bone and cartilage tissue engineering. Here we tested two non-standard ones, HE800 and GY785, for the two-dimensional (2-D) and three-dimensional (3-D) culture of osteoblasts (MC3T3-E1) and chondrocytes (C28/I2). These two glycosaminoglycan-like marine exopolysaccharides were incorporated into an injectable silylated hydroxypropylmethylcellulose-based hydrogel (Si-HPMC) that has already shown its suitability for bone and cartilage tissue engineering. Results showed that, similarly to hyaluronic acid (HA) (the control), HE800 and GY785 significantly improved the mechanical properties of the Si-HPMC hydrogel and induced the attachment of MC3T3-E1 and C28/I2 cells when these were cultured on top of the scaffolds. Si-HPMC hydrogel containing 0.67% HE800 exhibited the highest compressive modulus (11kPa) and allowed the best cell dispersion, especially of MC3T3-E1 cells. However, these cells did not survive when cultured in 3-D within hydrogels containing HE800, in contrast to C28/I2 cells. The latter proliferated in the microenvironment or concentrically depending on the nature of the hydrogel. Among all the constructs tested the Si-HPMC hydrogels containing 0.34% HE800 or 0.67% GY785 or 0.67% HA presented the most interesting features for cartilage tissue engineering applications, since they offered the highest compressive modulus (9.5-11kPa) while supporting the proliferation of chondrocytes.
机译：天然多糖是有吸引力的化合物，可用于构建骨骼和软骨组织工程的支架。在这里，我们测试了两种非标准的HE800和GY785，用于成骨细胞（MC3T3-E1）和软骨细胞（C28 / I2）的二维（2-D）和三维（3-D）培养。将这两种类似糖胺聚糖的海洋外多糖掺入可注射的甲硅烷基化羟丙基甲基纤维素基水凝胶（Si-HPMC）中，该凝胶已显示出其适用于骨骼和软骨组织工程的能力。结果表明，与透明质酸（HA）相似（对照），HE800和GY785显着改善了Si-HPMC水凝胶的机械性能，并在将MC3T3-E1和C28 / I2细胞培养在冰原上时诱导了它们的附着。脚手架。含0.67％HE800的Si-HPMC水凝胶表现出最高的压缩模量（11kPa），并具有最佳的细胞分散性，尤其是MC3T3-E1细胞。但是，与C28 / I2细胞相比，这些细胞在含HE800的水凝胶中以3-D培养时不能存活。后者在微环境中或根据水凝胶的性质同心扩散。在所有测试的构建体中，含有0.34％HE800或0.67％GY785或0.67％HA的Si-HPMC水凝胶为软骨组织工程应用提供了最有趣的功能，因为它们提供了最高的压缩模量（9.5-11kPa），同时支持了VEGF的增殖。软骨细胞。

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