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首页> 外文期刊>Acta biomaterialia >Enhanced immobilization of acidic proteins in the apatite layer via electrostatic interactions in a supersaturated calcium phosphate solution.
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Enhanced immobilization of acidic proteins in the apatite layer via electrostatic interactions in a supersaturated calcium phosphate solution.

机译：通过在过饱和磷酸钙溶液中的静电相互作用，增强了磷灰石层中酸性蛋白质的固定化。

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摘要

Artificial materials coated with a protein-apatite composite layer have great potential in clinical applications as a third generation biomaterial. Such composite materials can be prepared by immersing a surface modified substrate into a supersaturated calcium phosphate solution (CP solution: 142 mM NaCl, 3.75 mM CaCl(2), 1.5mM K(2)HPO(4).3H(2)O, buffered at pH 7.4 at 25 degrees C with tris(hydroxymethyl)aminomethane and HCl) supplemented with a protein. In the present study proteins of various molecular weights (MW) and isoelectric points (pI) were used to form a protein-apatite composite layer on a polymeric material to determine how the molecular properties of the protein affect the efficiency of protein immobilization (i.e. the amount of immobilized protein in the apatite layer as a percentage of the total amount of protein in solution). The results indicated that the efficiency of protein immobilization did not correlate with the MW of the protein. In contrast, the efficiency of protein immobilization was strongly related to the pI of the protein. As the pI decreased the efficiency of protein immobilization increased due to the high adsorption affinity of negatively charged acidic proteins for positively charged apatite crystals and/or apatite precursors in the CP solution. Thus, the use of acidic rather than basic proteins improves the immobilization efficiency in the present coating process.
机译：涂覆有蛋白质-磷灰石复合层的人工材料作为第三代生物材料在临床应用中具有巨大潜力。可以通过将表面改性的基材浸入过饱和的磷酸钙溶液中（CP溶液：142 mM NaCl，3.75 mM CaCl（2），1.5mM K（2）HPO（4）.3H（2）O，在25摄氏度下用补充蛋白质的三（羟甲基）氨基甲烷和HCl缓冲在pH 7.4。在本研究中，使用各种分子量（MW）和等电点（pI）的蛋白质在聚合物材料上形成蛋白质-磷灰石复合层，以确定蛋白质的分子特性如何影响蛋白质固定化的效率（即磷灰石层中固定化蛋白质的量占溶液中蛋白质总量的百分比）。结果表明，蛋白质固定化的效率与蛋白质的分子量无关。相反，蛋白质固定化的效率与蛋白质的pI密切相关。随着pI的降低，由于带负电的酸性蛋白质对CP溶液中带正电的磷灰石晶体和/或磷灰石前体的高吸附亲和力，蛋白质固定的效率提高了。因此，在本发明的包被方法中，使用酸性而不是碱性蛋白可以提高固定效率。

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