• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta biomaterialia >A crimp-like microarchitecture improves tissue production in fibrous ligament scaffolds in response to mechanical stimuli
【24h】

A crimp-like microarchitecture improves tissue production in fibrous ligament scaffolds in response to mechanical stimuli

机译：卷曲状的微结构可响应机械刺激提高纤维韧带支架的组织生产

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of this study was to determine the influence of a crimp-like microarchitecture within electrospun polymer scaffolds on fibroblast extracellular matrix (ECM) production when cultured under dynamic conditions. Electrospun poly(l-lactide-co-d,l-lactide) scaffolds possessing a wave pattern similar to collagen crimp (amplitude: 5 μm and wavelength: 46 μm) were seeded with bovine fibroblasts and mechanically stimulated under dynamic uniaxial tension. The effect of strain amplitude (5%, 10% and 20%) was investigated in a short-term stimulation study. The 10% strain amplitude in the stimulated crimp-like fibre scaffold increased only collagen synthesis, while the 20% strain amplitude increased both collagen and sulphated proteoglycan synthesis compared to stimulated uncrimped (straight) fibre scaffolds and unloaded controls (crimp-like static fibre scaffolds). Alternatively, mechanical stimulation of fibroblasts seeded on uncrimped fibre scaffolds induced significant fibroblast proliferation compared to the stimulated crimp-like fibre scaffolds and no-load controls. Long-term, dynamic mechanical stimulation of fibroblasts seeded on crimp-like fibre scaffolds at 10% strain amplitude resulted in significantly up-regulated collagen accumulation and down-regulated sulphated proteoglycan accumulation. Additionally, the fibroblasts seeded on dynamically stimulated crimp-like fibre scaffolds appeared to form bundles that resembled fascicles, a characteristic hierarchical feature of the native ligament. Our findings demonstrate that fibroblasts seeded on crimp-like fibrous scaffolds respond more favourably (increased ECM synthesis and fascicle formation) to dynamic mechanical loading compared to those grown on scaffolds containing uncrimped (straight) fibres.
机译：这项研究的目的是确定在动态条件下培养时，电纺聚合物支架内的卷曲状微体系结构对成纤维细胞细胞外基质（ECM）产生的影响。将具有类似于胶原卷曲（振幅：5μm，波长：46μm）的波形的静电纺丝聚（l-丙交酯-co-d，l-丙交酯）支架接种牛成纤维细胞，并在动态单轴张力下进行机械刺激。在短期刺激研究中研究了应变幅度（5％，10％和20％）的影响。与刺激的未卷曲（直形）纤维支架和空载对照（卷曲的静态纤维支架）相比，受刺激的卷曲状纤维支架中10％的应变幅度仅增加了胶原蛋白的合成，而受刺激的卷曲状纤维支架中的20％应变幅度均增加了胶原蛋白和硫酸化蛋白聚糖的合成。 ）。或者，与刺激的卷曲状纤维支架和空载对照相比，对未卷曲纤维支架上播种的成纤维细胞进行机械刺激可诱导显着的成纤维细胞增殖。长期动态机械刺激以10％的应变幅度接种在卷曲状纤维支架上的成纤维细胞会导致胶原蛋白的积累明显上调，而硫酸化蛋白聚糖的积累则下降。另外，接种在动态刺激的卷曲状纤维支架上的成纤维细胞似乎形成了类似于束的束，这是天然韧带的特征性分层特征。我们的发现表明，与在包含未卷曲（直形）纤维的支架上生长的成纤维细胞相比，接种在卷曲状纤维支架上的成纤维细胞对动态机械负载的响应更佳（ECM合成和纤维束形成增加）。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号