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首页> 外文期刊>Acta biomaterialia >Analysis of hydrolyzable polyethylene glycol hydrogels and deproteinized bone mineral as delivery systems for glycosylated and non-glycosylated bone morphogenetic protein-2
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Analysis of hydrolyzable polyethylene glycol hydrogels and deproteinized bone mineral as delivery systems for glycosylated and non-glycosylated bone morphogenetic protein-2

机译：水解聚乙二醇水凝胶和去蛋白骨矿物质作为糖基化和非糖基化骨形态发生蛋白2的递送系统的分析

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摘要

Bone morphogenetic proteins (BMP), in particular BMP-2, are the growth factors primarily responsible for osteoinduction. A knowledge of interactions between bone substitute materials and growth factor variants is crucial to designing bone substitutes with an ideal release profile. Here we compare glycosylated and non-glycosylated recombinant human bone morphogenetic protein-2 (rhBMP-2) either incorporated into a hydrolyzable polyethylene glycol (PEG) hydrogel developed as a slow release system or adsorbed to a deproteinized bovine bone matrix (DBBM), a clinically well-established bone substitute material. rhBMP-2 loaded materials were immersed in cell culture medium and rhBMP-2 concentration profiles in the supernatant were determined by an enzyme-linked immunosorbent assay. The corresponding biological activities were assessed in vitro by alkaline phosphatase activity assay. We show a strong affinity of rhBMP-2 for DBBM and reduced biological activity after its release from PEG hydrogels. Glycosylated rhBMP-2 was significantly less affected by the hydrogel and interacted significantly more strongly with DBBM than non-glycosylated rhBMP-2. We therefore question the combination of PEG hydrogels with DBBM as a rhBMP-2 delivery system over DBBM alone, since rhBMP-2 released from the hydrogel will be trapped by DBBM. Moreover, our results suggest that glycosylated rhBMP-2 is favorable in combination with PEG hydrogels, since its activity is better preserved, whereas in combination with DBBM non-glycosylated rhBMP-2 is favorable, benefiting from an initially higher concentration of free rhBMP-2.
机译：骨形态发生蛋白（BMP），特别是BMP-2，是主要负责骨诱导的生长因子。骨替代材料与生长因子变体之间相互作用的知识对于设计具有理想释放曲线的骨替代品至关重要。在这里，我们比较糖基化和非糖基化的重组人骨形态发生蛋白2（rhBMP-2），将其掺入开发为缓慢释放系统的可水解聚乙二醇（PEG）水凝胶中，或吸附到脱蛋白的牛骨基质（DBBM）中，临床上公认的骨替代材料。将装有rhBMP-2的材料浸入细胞培养基中，并通过酶联免疫吸附试验确定上清液中rhBMP-2的浓度。通过碱性磷酸酶活性测定在体外评估相应的生物学活性。我们显示出rhBMP-2对DBBM具有很强的亲和力，并从PEG水凝胶中释放后降低了生物活性。糖基化的rhBMP-2与非糖基化的rhBMP-2相比，受水凝胶的影响明显较小，并且与DBBM的相互作用明显更强。因此，我们对PEG水凝胶与DBBM的结合作为单独的DBBM上的rhBMP-2传递系统提出质疑，因为从水凝胶中释放的rhBMP-2将被DBBM捕获。此外，我们的结果表明，糖基化的rhBMP-2与PEG水凝胶结合使用是有利的，因为它的活性得到更好的保存，而与DBBM的非糖基化的rhBMP-2结合则是有利的，这得益于最初浓度较高的游离rhBMP-2 。

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