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首页> 外文期刊>Acta biomaterialia >Human corneal limbal epithelial cell response to varying silk film geometric topography in vitro
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Human corneal limbal epithelial cell response to varying silk film geometric topography in vitro

机译：人角膜缘上皮细胞对体外丝膜几何形貌的反应

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摘要

Silk fibroin films are a promising class of biomaterials that have a number of advantages for use in ophthalmic applications due to their transparent nature, mechanical properties and minimal inflammatory response upon implantation. Freestanding silk films with parallel line and concentric ring topographies were generated for in vitro characterization of human corneal limbal epithelial (HCLE) cell response upon differing geometric patterned surfaces. Results indicated that silk film topography significantly affected initial HCLE culture substrate attachment, cellular alignment, cell-to-cell contact formation, actin cytoskeleton alignment and focal adhesion (FA) localization. Most notably, parallel line patterned surfaces displayed a 36-54% increase on average in initial cell attachment, which corresponded to a more than 2-fold increase in FA localization when compared to other silk film surfaces and controls. In addition, distinct localization of FA formation was observed along the edges for all patterned silk film topographies. In conclusion, silk film feature topography appears to help direct corneal epithelial cell response and cytoskeleton development, especially with regard to FA distribution, in vitro.
机译：丝素蛋白膜是一类有前途的生物材料，由于其透明性，机械性能和植入后的最小炎症反应，在眼科应用中具有许多优势。产生具有平行线和同心环形貌的独立式丝膜，用于体外表征在不同几何图案表面上的人角膜缘角膜上皮（HCLE）细胞反应。结果表明，丝膜形貌显着影响了最初的HCLE培养底物附着，细胞排列，细胞间接触形成，肌动蛋白细胞骨架排列和粘着性（FA）定位。最值得注意的是，平行线图案化的表面在初始细胞附着上显示出平均36-54％的增加，与其他丝膜表面和对照相比，其FA定位增加了2倍以上。另外，对于所有图案化的丝膜形貌，沿着边缘观察到FA形成的独特定位。总之，丝膜特征形貌似乎在体外有助于指导角膜上皮细胞反应和细胞骨架发育，特别是在FA分布方面。

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