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首页> 外文期刊>Acta biomaterialia >The identity of the cell adhesive protein substrate affects the efficiency of adeno-associated virus reverse transduction.
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The identity of the cell adhesive protein substrate affects the efficiency of adeno-associated virus reverse transduction.

机译：细胞粘附蛋白底物的身份影响腺相关病毒反向转导的效率。

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摘要

Delivering genes from surfaces, called substrate-mediated gene delivery or reverse transduction, is a useful method to achieve spatial localization of gene delivery. We tested the compatibility of adeno-associated virus (AAV) vectors with various cell adhesive proteins to mediate gene delivery from surfaces. Our studies demonstrate that AAV vectors can be successfully adsorbed on collagen I, elastin, and laminin substrates leading to robust gene delivery to overlying cells. Notably, AAV immobilization on laminin yields the highest efficiency of gene expression. This increased gene expression cannot be explained by increases in the levels of virus deposition, transcriptional activity of cells, or virus vector uptake into cells. Further refinement of our knowledge of AAV interactions with extracellular matrix proteins may have important implications in a variety of applications ranging from tissue engineering to in vivo gene therapy.
机译：从表面传递基因，称为底物介导的基因传递或反向转导，是实现基因传递空间定位的有用方法。我们测试了腺相关病毒（AAV）载体与各种细胞粘附蛋白介导从表面传递基因的相容性。我们的研究表明，AAV载体可以成功吸附在I型胶原蛋白，弹性蛋白和层粘连蛋白底物上，从而将强大的基因传递至上层细胞。值得注意的是，将AAV固定在层粘连蛋白上可产生最高的基因表达效率。不能通过病毒沉积水平，细胞转录活性或病毒载体吸收进入细胞的水平来解释这种基因表达的增加。进一步完善我们对AAV与细胞外基质蛋白相互作用的认识可能对从组织工程到体内基因治疗的各种应用产生重要影响。

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