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首页> 外文期刊>Acta biomaterialia >The role of fibrinogen spacing and patch size on platelet adhesion under flow.
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The role of fibrinogen spacing and patch size on platelet adhesion under flow.

机译：纤维蛋白原的间距和斑块大小对流动下血小板粘附的作用。

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Platelet adhesion to the vessel wall during vascular injury is mediated by platelet glycoproteins binding to their respective ligands on the vascular wall. In this study we investigated the roles that ligand patch spacing and size play in regulating platelet interactions with fibrinogen under hemodynamic flow conditions. To regulate the size and distance between patches of fibrinogen we developed a photolithography-based technique to fabricate patterns of proteins surrounded by a protein-repellant layer of poly(ethylene glycol). We demonstrate that when mepacrine labeled whole blood is perfused at a shear rate of 100 s ?1 over substrates patterned with micron-sized wide lines of fibrinogen, platelets selectively adhere to the areas of patterned fibrinogen. Using fluorescent and scanning electron microscopy we demonstrate that the degree of platelet coverage (3-35%) and the ability of platelet aggregates to grow laterally are dependent upon the distance (6-30 μm) between parallel lines of fibrinogen. We also report on the effects of fibrinogen patch size on platelet adhesion by varying the size of the protein patch (2-20 μm) available for adhesion, demonstrating that the downstream length of the ligand patch is a critical parameter in platelet adhesion under flow. We expect that these results and protein patterning surfaces will be useful in understanding the spatial and temporal dynamics of platelet adhesion under physiologic flow, and in the development of novel platelet adhesion assays.
机译：血小板在血管损伤期间对血管壁的粘附是通过血小板糖蛋白与其在血管壁上的各自配体的结合来介导的。在这项研究中，我们研究了在血流动力学条件下，配体斑块间距和大小在调节血小板与纤维蛋白原相互作用中的作用。为了调节纤维蛋白原补丁之间的大小和距离，我们开发了一种基于光刻的技术来制造被聚乙二醇的蛋白质排斥层包围的蛋白质图案。我们证明，当用米卡培林标记的全血以100 s？1的剪切速率在具有微米级纤维蛋白原的宽线条图案的基质上灌注时，血小板选择性地附着在图案化纤维蛋白原的区域。使用荧光和扫描电子显微镜，我们证明了血小板覆盖度（3％至35％）和血小板聚集体横向生长的能力取决于纤维蛋白原平行线之间的距离（6-30μm）。我们还通过改变可用于粘附的蛋白质补丁的大小（2-20μm）来报告纤维蛋白原补丁大小对血小板粘附的影响，表明配体补丁的下游长度是流动条件下血小板粘附的关键参数。我们希望这些结果和蛋白质构图表面将有助于理解生理流下血小板粘附的时空动态，并有助于新型血小板粘附测定方法的发展。

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