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首页> 外文期刊>Acta biomaterialia >Adipose stem cell-derived osteoblasts sustain the functionality of endothelial progenitors from the mononuclear fraction of umbilical cord blood
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Adipose stem cell-derived osteoblasts sustain the functionality of endothelial progenitors from the mononuclear fraction of umbilical cord blood

机译：脂肪干细胞来源的成骨细胞通过脐带血的单核部分维持内皮祖细胞的功能

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Vascularization is the most pressing issue in tissue engineering (TE) since ensuring that engineered constructs are adequately perfused after in vivo transplantation is essential for the construct's survival. The combination of endothelial cells with current TE strategies seems the most promising approach but doubts persist as to which type of endothelial cells to use. Umbilical cord blood (UCB) cells have been suggested as a possible source of endothelial progenitors. Osteoblasts obtained from human adipose-derived stem cells (hASCs) were co-cultured with the mononuclear fraction of human UCB for 7 and 21 days on carrageenan membranes. The expression of vWF and CD31, and the DiI-AcLDL uptake ability allowed detection of the presence of endothelial and monocytic lineages cells in the co-culture for all culture times. In addition, the molecular expression of CD31 and VE-cadherin increased after 21 days of co-culture. The functionality of the system was assessed after transplantation in nude mice. Although an inflammatory response developed, blood vessels with cells positive for human CD31 were detected around the membranes. Furthermore, the number of blood vessels in the vicinity of the implants increased when cells from the mononuclear fraction of UCB were present in the transplants compared to transplants with only hASC-derived osteoblasts. These results show how endothelial progenitors present in the mononuclear fraction of UCB can be sustained by hASC-derived osteoblast co-culture and contribute to angiogenesis even in an in vivo setting of inflammatory response. ? 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
机译：血管化是组织工程（TE）中最紧迫的问题，因为确保在体内移植后对工程化的构建体进行充分灌注对于构建体的生存至关重要。将内皮细胞与当前的TE策略结合起来似乎是最有前途的方法，但是对于使用哪种类型的内皮细胞仍存在疑问。脐带血（UCB）细胞已被建议作为内皮祖细胞的可能来源。将得自人类脂肪干细胞（hASC）的成骨细胞与人类UCB的单核级分在角叉菜胶膜上共培养7天和21天。 vWF和CD31的表达以及DiI-AcLDL摄取能力允许在所有培养时间内检测共培养物中内皮细胞和单核细胞谱系细胞的存在。另外，共培养21天后，CD31和VE-钙粘着蛋白的分子表达增加。在裸鼠中移植后评估系统的功能。尽管发生了炎症反应，但在膜周围发现了具有人类CD31阳性细胞的血管。此外，与仅由hASC衍生的成骨细胞移植相比，当移植物中存在来自UCB单核部分的细胞时，移植物附近的血管数量增加。这些结果表明，通过hASC衍生的成骨细胞共培养物，UCB单核级分中存在的内皮祖细胞如何得以维持，甚至在体内炎症反应的情况下也有助于血管生成。 ？ 2012年Acta Materialia Inc.由Elsevier Ltd.发行。保留所有权利。

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