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首页> 外文期刊>Acta biomaterialia >Collagen hydrogels incorporated with surface-aminated mesoporous nanobioactive glass: Improvement of physicochemical stability and mechanical properties is effective for hard tissue engineering
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Collagen hydrogels incorporated with surface-aminated mesoporous nanobioactive glass: Improvement of physicochemical stability and mechanical properties is effective for hard tissue engineering

机译：结合了表面胺化介孔纳米生物活性玻璃的胶原蛋白水凝胶：物理化学稳定性和机械性能的改善对硬组织工程有效

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摘要

Collagen (Col) hydrogels have poor physicochemical and mechanical properties and are susceptible to substantial shrinkage during cell culture, which limits their potential applications in hard tissue engineering. Here, we developed novel nanocomposite hydrogels made of collagen and mesoporous bioactive glass nanoparticles (mBGns) with surface amination, and addressed the effects of mBGn addition (Col:mBG = 2:1, 1:1 and 1:2) and its surface amination on the physicochemical and mechanical properties of the hydrogels. The amination of mBGn was shown to enable chemical bonding with collagen molecules. As a result, the nanocomposite hydrogels exhibited a significantly improved physicochemical and mechanical stability. The hydrolytic and enzymatic degradation of the Col-mBGn hydrogels were slowed down due to the incorporation of mBGn and its surface amination. The mechanical properties of the hydrogels, specifically the resistance to loading as well as the stiffness, significantly increased with the addition of mBGn and its aminated form, as assessed by a dynamic mechanical analysis. Mesenchymal stem cells cultivated within the Col-mBGn hydrogels were highly viable, with enhanced cytoskeletal extensions, due to the addition of surface aminated mBGn. While the Col hydrogel showed extensive shrinkage (down to ～20% of initial size) during a few days of culture, the shrinkage of the mBGn-added hydrogel was substantially reduced, and the aminated mBGn-added hydrogel had no observable shrinkage over 21 days. Results demonstrated the effective roles of aminated mBGn in significantly improving the physicochemical and mechanical properties of Col hydrogel, which are ultimately favorable for applications in stem cell culture for bone tissue engineering.
机译：胶原蛋白（Col）水凝胶的理化性质和机械性质较差，并且在细胞培养过程中容易收缩，这限制了其在硬组织工程中的潜在应用。在这里，我们开发了由具有表面胺化作用的胶原蛋白和中孔生物活性玻璃纳米颗粒（mBGns）制成的新型纳米复合水凝胶，并解决了添加mBGn（Col：mBG = 2：1、1：1和1：2）及其表面胺化的影响关于水凝胶的理化和力学性能。显示出mBGn的胺化能够与胶原分子化学键合。结果，纳米复合水凝胶表现出显着改善的物理化学和机械稳定性。由于mBGn的掺入及其表面胺化作用，Col-mBGn水凝胶的水解和酶促降解速度减慢。通过动态力学分析评估，加入mBGn及其胺化形式后，水凝胶的机械性能，特别是抗负载性和刚度显着提高。由于添加了表面胺化的mBGn，在Col-mBGn水凝胶中培养的间充质干细胞具有很高的生存能力，具有增强的细胞骨架延伸性。虽然Col水凝胶在培养的几天中显示出广泛的收缩（下降至初始尺寸的约20％），但添加了mBGn的水凝胶的收缩却显着减少，并且胺化的添加了mBGn的水凝胶在21天之内没有观察到收缩。 。结果证明了胺化的mBGn在显着改善Col水凝胶的物理化学和机械性能方面的有效作用，这最终有利于在骨组织工程的干细胞培养中的应用。

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