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首页> 外文期刊>Acta biomaterialia >Biochemical changes caused by decellularization may compromise mechanical integrity of tracheal scaffolds
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Biochemical changes caused by decellularization may compromise mechanical integrity of tracheal scaffolds

机译：由脱细胞作用引起的生化变化可能会损害气管支架的机械完整性

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摘要

Tissue-engineered airways have achieved clinical success, but concerns remain about short-term loss of biomechanical properties, necessitating a stent. This study investigated the effect of chemical-enzymatic decellularization on biochemical properties of trachea important for cell attachment and vascularization (fibronectin and laminin) and cartilage matrix homeostasis (type II collagen and glycosaminoglycans (GAG)), as well as biomechanical status. Native trachea was used as a control, and NDC trachea stored in phosphate buffered saline (PBS) in parallel to decellularization was used as a time-matched control. Decellularization removed most cells, but chondrocytes and DNA remained after 25 cycles. Fibronectin was retained throughout the lamina propria and laminin at basement membranes. DNA accumulation along ECM fibres was seen. A decline in soluble collagen was observed in decellularized tissue. GAG content of cartilage rings was reduced, even in PBS control tissue from 20 cycles onwards (p < 0.05), but decellularization caused the greatest loss (p < 0.01). Tensile strength declined throughout the process, but was significant only at later time points. The data demonstrate that the substantial reduction in GAG might contribute to loss of mechanical integrity of biotracheas. Overcoming structural changes that cause an imbalance in cartilage matrix equilibrium will be necessary to optimize clinical benefit, enabling widespread use of biotracheas. ? 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
机译：组织工程化气道已取得临床成功，但仍存在对短期丧失生物力学特性的担忧，因此需要使用支架。这项研究调查了化学酶脱细胞对气管生化特性的影响，这些特性对细胞附着和血管形成（纤连蛋白和层粘连蛋白）和软骨基质稳态（II型胶原蛋白和糖胺聚糖（GAG））很重要，并且具有生物力学状态。天然气管用作对照，NDC气管与脱细胞同时储存在磷酸盐缓冲盐水（PBS）中作为时间匹配的对照。脱细胞作用去除了大多数细胞，但在25个循环后仍保留了软骨细胞和DNA。纤连蛋白保留在整个固有层和层粘连蛋白的基底膜中。观察到沿ECM纤维的DNA积累。在去细胞的组织中观察到可溶性胶原蛋白的下降。从20个周期开始，即使在PBS对照组织中，软骨环的GAG含量也降低了（p <0.05），但是脱细胞引起的损失最大（p <0.01）。拉伸强度在整个过程中均下降，但仅在随后的时间点才显着。数据表明，GAG的大幅降低可能会导致气管机械完整性的丧失。克服导致软骨基质平衡失衡的结构改变对于优化临床利益是必要的，从而可以广泛使用生物气管。 ？ 2012年Acta Materialia Inc.由Elsevier Ltd.发行。保留所有权利。

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