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首页> 外文期刊>Acta biomaterialia >Bioengineered surfaces promote specific protein-glycan mediated binding of the gastric pathogen Helicobacter pylori
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Bioengineered surfaces promote specific protein-glycan mediated binding of the gastric pathogen Helicobacter pylori

机译：生物工程化的表面促进胃病原体幽门螺杆菌特异性蛋白聚糖介导的结合

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摘要

Helicobacter pylori colonizes the gastric mucosa of half of the worlds population and persistent infection is related with an increase in the risk of gastric cancer. Adhesion of H. pylori to the gastric epithelium, which is essential for infection, is mediated by bacterial adhesin proteins that recognize specific glycan structures (Gly-R) expressed in the gastric mucosa. The blood group antigen binding adhesin (BabA) recognizes difucosylated antigens such as Lewis B (Leb), while the sialic acid binding adhesin (SabA) recognizes sialylated glycoproteins and glycolipids, such as sialyl-Lewis x (sLex). This work aimed to investigate whether these Gly-Rs (Le b and sLex) can attract and specifically bind H. pylori after immobilization on synthetic surfaces (self-assembled monolayers (SAMs) of alkanethiols on gold). Functional bacterial adhesion assays for (Gly-R)-SAMs were performed using H. pylori strains with different adhesin protein profiles. The results demonstrate that H. pylori binding to surfaces occurs via interaction between its adhesins and cognate (Gly-R)-SAMs and bound H. pylori maintains its characteristic rod-shaped morphology only during conditions of specific adhesin-glycan binding. These results offer new insights into innovative strategies against H. pylori infection based on the scavenging of bacteria from the stomach using specific H. pylori chelating biomaterials.
机译：幽门螺杆菌定植在世界一半人口的胃粘膜中，持续感染与胃癌风险增加有关。幽门螺杆菌对胃上皮的粘附是感染必不可少的，它是由细菌粘附素蛋白介导的，该蛋白质识别在胃粘膜中表达的特定聚糖结构（Gly-R）。血型抗原结合粘附素（BabA）识别双岩藻糖基化抗原，例如Lewis B（Leb），而唾液酸结合粘附素（SabA）则识别唾液酸化的糖蛋白和糖脂，例如唾液酸化的刘易斯x（sLex）。这项工作旨在调查这些Gly-R（Le b和sLex）在固定在合成表面（链烷硫醇在金上的自组装单层（SAMs））上固定后是否能吸引并特异性结合幽门螺杆菌。 （Gly-R）-SAMs的功能性细菌粘附测定是使用具有不同粘附素蛋白谱的幽门螺杆菌菌株进行的。结果表明幽门螺杆菌与表面的结合是通过其粘附素与同源（Gly-R）-SAMs之间的相互作用而发生的，并且结合的幽门螺杆菌仅在特定粘附素-聚糖结合的条件下才保持其特征性的杆状形态。这些结果为基于使用特定幽门螺杆菌螯合生物材料从胃中清除细菌的幽门螺杆菌感染的创新策略提供了新见识。

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