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首页> 外文期刊>Acta biomaterialia >Effect of sterilization on structural and material properties of 3-D silk fibroin scaffolds
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Effect of sterilization on structural and material properties of 3-D silk fibroin scaffolds

机译：灭菌对3-D丝素蛋白支架结构和材料性能的影响

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The development of porous scaffolds for tissue engineering applications requires the careful choice of properties, as these influence cell adhesion, proliferation and differentiation. Sterilization of scaffolds is a prerequisite for in vitro culture as well as for subsequent in vivo implantation. The variety of methods used to provide sterility is as diverse as the possible effects they can have on the structural and material properties of the three-dimensional (3-D) porous structure, especially in polymeric or proteinous scaffold materials. Silk fibroin (SF) has previously been demonstrated to offer exceptional benefits over conventional synthetic and natural biomaterials in generating scaffolds for tissue replacements. This study sought to determine the effect of sterilization methods, such as autoclaving, heat-, ethylene oxide-, ethanol- or antibiotic-antimycotic treatment, on porous 3-D SF scaffolds. In terms of scaffold morphology, topography, crystallinity and short-term cell viability, the different sterilization methods showed only few effects. Nevertheless, mechanical properties were significantly decreased by a factor of two by all methods except for dry autoclaving, which seemed not to affect mechanical properties compared to the native control group. These data suggest that SF scaffolds are in general highly resistant to various sterilization treatments. Nevertheless, care should be taken if initial mechanical properties are of interest.
机译：用于组织工程应用的多孔支架的开发需要仔细选择特性，因为它们会影响细胞粘附，增殖和分化。支架的灭菌是体外培养以及随后体内植入的前提条件。用于提供无菌性的方法多种多样，它们可能会对三维（3-D）多孔结构的结构和材料特性产生可能的影响，尤其是在聚合物或蛋白质支架材料中。以前已经证明，丝素蛋白（SF）在产生用于组织替代的支架方面，比常规的合成和天然生物材料具有卓越的优势。这项研究试图确定灭菌方法（例如高压灭菌，热，环氧乙烷，乙醇或抗生素抗真菌剂处理）对多孔3-D SF支架的影响。在支架的形态，形貌，结晶度和短期细胞活力方面，不同的灭菌方法仅显示很少的作用。然而，除干式高压灭菌外，所有方法的机械性能均显着降低了两倍，与天然对照组相比似乎没有影响机械性能。这些数据表明，SF支架通常对各种灭菌处理具有高度的抵抗力。但是，如果要关注初始机械性能，则应格外小心。

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