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首页> 外文期刊>Acta biomaterialia >Degradation in the dentin-composite interface subjected to multi-species biofilm challenges
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Degradation in the dentin-composite interface subjected to multi-species biofilm challenges

机译：牙本质-复合材料界面降解受到多种生物膜挑战

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Oral biofilms can degrade the components in dental resin-based composite restorations, thus compromising marginal integrity and leading to secondary caries. This study investigates the mechanical integrity of the dentin-composite interface challenged with multi-species oral biofilms. While most studies used single-species biofilms, the present study used a more realistic, diverse biofilm model produced directly from plaques collected from donors with a history of early childhood caries. Dentin-composite disks were made using bovine incisor roots filled with Z100TM or FiltekTM LS (3M ESPE). The disks were incubated for 72 h in paired CDC biofilm reactors, using a previously published protocol. One reactor was pulsed with sucrose, and the other was not. A sterile saliva-only control group was run with sucrose pulsing. The disks were fractured under diametral compression to evaluate their interfacial bond strength. The surface deformation of the disks was mapped using digital image correlation to ascertain the fracture origin. Fracture surfaces were examined using scanning electron microscopy/energy-dispersive X-ray spectroscopy to assess demineralization and interfacial degradation. Dentin demineralization was greater under sucrose-pulsed biofilms, as the pH dropped 5.5 during pulsing, with LS and Z100 specimens suffering similar degrees of surface mineral loss. Biofilm growth with sucrose pulsing also caused preferential degradation of the composite-dentin interface, depending on the composite/adhesive system used. Specifically, Z100 specimens showed greater bond strength reduction and more frequent cohesive failure in the adhesive layer. This was attributed to the inferior dentin coverage by Z100 adhesive, which possibly led to a higher level of chemical and enzymatic degradation. The results suggested that factors other than dentin demineralization were also responsible for interfacial degradation. A clinically relevant in vitro biofilm model was therefore developed, which would effectively allow assessment of the degradation of the dentin-composite interface subjected to multi-species biofilm challenge.
机译：口腔生物膜可降解牙科树脂基复合修复物中的成分，从而损害边缘完整性并导致继发龋齿。这项研究调查了多物种口腔生物膜挑战牙本质-复合材料界面的机械完整性。虽然大多数研究使用的是单一物种的生物膜，但本研究使用的是更现实，更多样化的生物膜模型，该模型直接从具有早期龋齿历史的捐赠者的斑块中产生。使用填充有Z100TM或FiltekTM LS（3M ESPE）的牛切牙根制成牙本质复合盘。使用先前公布的协议，将磁盘在成对的CDC生物膜反应器中孵育72小时。一个反应器用蔗糖脉冲，而另一个则不。仅无菌唾液对照组进行蔗糖脉动。圆盘在径向压缩下断裂，以评估其界面粘结强度。使用数字图像相关性绘制圆盘的表面变形图，以确定断裂起点。使用扫描电子显微镜/能量分散X射线光谱仪检查骨折表面，以评估脱矿质和界面降解。在蔗糖脉冲生物膜下，牙本质的脱矿质作用更大，因为在脉冲过程中pH值降低至<5.5，而LS和Z100标本遭受相似程度的表面矿物质流失。取决于蔗糖脉冲的生物膜生长也导致复合物-牙本质界面的优先降解，这取决于所使用的复合物/粘合剂系统。具体而言，Z100样品在粘合层中表现出更大的粘合强度降低和更频繁的内聚破坏。这归因于Z100粘合剂对牙本质的覆盖力较差，这可能导致更高的化学和酶降解水平。结果表明，除牙本质脱矿质之外的其他因素也可导致界面降解。因此，开发了一种临床相关的体外生物膜模型，该模型将有效地评估遭受多种生物膜攻击的牙本质-复合材料界面的降解情况。

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