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首页> 外文期刊>Acta biomaterialia >Cytotoxicity evaluation of nanocrystalline diamond coatings by fibroblast cell cultures.
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Cytotoxicity evaluation of nanocrystalline diamond coatings by fibroblast cell cultures.

机译：通过成纤维细胞细胞培养评估纳米晶金刚石涂层的细胞毒性。

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摘要

The cytotoxicity profile of nanocrystalline diamond (NCD) coatings on a Si(3)N(4) ceramic was investigated. This material is envisaged to have biomedical dental applications such as burrs and surgical instruments. Two fibroblast cell culture systems were used to address the cytotoxicity of NCD-coated samples: L929 cells (a mouse permanent cell line) and human gingival fibroblasts. Cell behavior was evaluated in terms of cell adhesion, cell viability/proliferation (mitochondrial function, MTT assay) and the pattern of cell growth. Fibroblast cell behavior on standard polystyrene culture plates was used as control, as Si(3)N(4) substrates have previously been shown to be biocompatible. NCD coatings provided a suitable surface for cell attachment, spreading and proliferation. Human gingival cells showed a homogeneous cytoplasm spreading, a flattened elongated morphology and a typical parallel alignment on confluent cultures. In comparison, L929 cells denoted a lower cytoplasm expansion, a heterogeneous spreading but a higher proliferation rate. For both cells, after few days, the NCD coating was completely covered with continuous cell layers. As compared to standard polystyrene culture plates, no deleterious or cytotoxic responses were observed with L929 and human fibroblast cell cultures, and in both a slight enhancement in cell proliferation was observed. In addition, the seeded NCD film allowed reproduction of the typical features of the two cell culture systems tested, further suggesting the lack of cytotoxicity of this coating.
机译：研究了Si（3）N（4）陶瓷上的纳米晶金刚石（NCD）涂层的细胞毒性特征。设想该材料具有生物医学牙科应用，例如毛刺和手术器械。两种成纤维细胞培养系统用于解决NCD包被样品的细胞毒性：L929细胞（小鼠永久细胞系）和人牙龈成纤维细胞。根据细胞粘附，细胞活力/增殖（线粒体功能，MTT测定）和细胞生长方式评估细胞行为。由于以前已显示Si（3）N（4）底物具有生物相容性，因此将标准聚苯乙烯培养板上的成纤维细胞行为用作对照。 NCD涂层为细胞附着，扩散和增殖提供了合适的表面。人牙龈细胞在融合培养物中显示出均匀的细胞质散布，扁平的细长形态和典型的平行排列。相比之下，L929细胞表现出较低的细胞质扩展，异质扩散但具有较高的增殖率。对于两个电池，几天后，NCD涂层被连续的细胞层完全覆盖。与标准聚苯乙烯培养板相比，L929和人成纤维细胞培养物未观察到有害或细胞毒性反应，并且两者均观察到细胞增殖略有增强。另外，接种的NCD膜可再现所测试的两种细胞培养系统的典型特征，进一步表明该涂层缺乏细胞毒性。

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