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首页> 外文期刊>Acta biomaterialia >Spatially controlled bacterial adhesion using surface-patterned poly(ethylene glycol) hydrogels.
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Spatially controlled bacterial adhesion using surface-patterned poly(ethylene glycol) hydrogels.

机译：使用表面图案化的聚乙二醇水凝胶在空间上控制细菌粘附。

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摘要

We constructed surface-patterned hydrogels using low-energy focused electron beams to locally crosslink poly(ethylene glycol) (PEG) thin films on silanized glass substrates. Derived from electron-beam lithography, this technique was used to create patterned hydrogels with well-defined spatial positions and degrees of swelling. We found that cells of the bacterium Staphylococcus epidermidis adhered to and grew on the silanized glass substrates. These cells did not, however, adhere to surfaces covered by high-swelling lightly crosslinked PEG hydrogels. This finding is consistent with the cell-repulsiveness generally attributed to PEGylated surfaces. In contrast, S. epidermidis cells did adhere to surfaces covered by low-swelling highly crosslinked hydrogels. By creating precise patterns of repulsive hydrogels combined with adhesive hydrogels or with exposed glass substrate, we were able to spatially control the adhesion of S. epidermidis. Significantly, adhesive areas small enough to trap single bacterial cells could be fabricated. The results suggest that the lateral confinement imposed by cell-repulsive hydrogels hindered the cell proliferation and development into larger bacterial colonies.
机译：我们使用低能量聚焦电子束构造表面图案化水凝胶，以在硅烷化玻璃基板上局部交联聚（乙二醇）（PEG）薄膜。源自电子束光刻技术，该技术用于创建具有明确定义的空间位置和溶胀度的图案化水凝胶。我们发现细菌表皮葡萄球菌的细胞粘附并在硅烷化的玻璃基板上生长。但是，这些细胞没有粘附到被高溶胀的轻度交联的PEG水凝胶覆盖的表面上。这一发现与通常归因于聚乙二醇化表面的细胞排斥性是一致的。相反，表皮葡萄球菌细胞确实粘附在低膨胀高度交联的水凝胶覆盖的表面上。通过创建与粘合水凝胶或裸露的玻璃基板相结合的排斥水凝胶的精确图案，我们能够在空间上控制表皮葡萄球菌的粘附。值得注意的是，可以制造足够小以捕获单个细菌细胞的粘合区域。结果表明，排斥细胞的水凝胶所施加的横向限制阻碍了细胞的增殖和发展成更大的细菌菌落。

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