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首页> 外文期刊>Acta biomaterialia >The use of layer by layer self-assembled coatings of hyaluronic acid and cationized gelatin to improve the biocompatibility of poly(ethylene terephthalate) artificial ligaments for reconstruction of the anterior cruciate ligament.
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The use of layer by layer self-assembled coatings of hyaluronic acid and cationized gelatin to improve the biocompatibility of poly(ethylene terephthalate) artificial ligaments for reconstruction of the anterior cruciate ligament.

机译：透明质酸和阳离子明胶的逐层自组装涂层的使用，以改善聚对苯二甲酸乙二酯人造韧带的生物相容性，以重建前十字韧带。

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摘要

In this study layer by layer (LBL) self-assembled coatings of hyaluronic acid (HA) and cationized gelatin (CG) were used to modify polyethylene terephthalate (PET) artificial ligament grafts. Changes in the surface properties were characterized by scanning electron microscopy, attenuated total reflection Fourier transform infrared spectroscopy, energy-dispersive X-ray spectroscopy, and contact angle and biomechanical measurements. The cell compatibility of this HA-CG coating was investigated in vitro on PET films seeded with human foreskin dermal fibroblasts over 7days. The results of our in vitro studies demonstrated that the HA-CG coating significantly enhanced cell adhesion, facilitated cell growth, and suppressed the expression of inflammation-related genes relative to a pure PET graft. Furthermore, rabbit and porcine anterior cruciate ligament reconstruction models were used to evaluate the effect of this LBL coating in vivo. The animal experiment results proved that this LBL coating significantly inhibited inflammatory cell infiltration and promoted new ligament tissue regeneration among the graft fibers. In addition, the formation of type I collagen in the HA-CG coating group was much higher than in the control group. Based on these results we conclude that PET grafts coated with HA-CG have considerable potential as substitutes for ligament reconstruction.
机译：在这项研究中，透明质酸（HA）和阳离子明胶（CG）的自组装涂层被用于修饰聚对苯二甲酸乙二醇酯（PET）人造韧带移植物。通过扫描电子显微镜，衰减全反射傅立叶变换红外光谱，能量色散X射线光谱以及接触角和生物力学测量来表征表面性质的变化。在7天的时间内，在接种人包皮真皮成纤维细胞的PET薄膜上体外研究了该HA-CG涂层的细胞相容性。我们的体外研究结果表明，相对于纯PET移植，HA-CG涂层显着增强了细胞粘附力，促进了细胞生长，并抑制了炎症相关基因的表达。此外，兔和猪前交叉韧带重建模型用于评估该LBL涂层在体内的效果。动物实验结果证明，这种LBL涂层显着抑制了炎症细胞的浸润并促进了移植纤维之间新的韧带组织再生。另外，HA-CG涂层组中I型胶原蛋白的形成远高于对照组。根据这些结果，我们得出结论，用HA-CG涂层的PET移植物具有相当大的潜力，可以替代韧带重建术。

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