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首页> 外文期刊>Acta biomaterialia >Submicron bioactive glass tubes for bone tissue engineering
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Submicron bioactive glass tubes for bone tissue engineering

机译：用于骨组织工程的亚微米生物活性玻璃管

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Herein we describe a method to fabricate submicron bioactive glass tubes using sol-gel and coaxial electrospinning techniques for applications in bone tissue engineering. Heavy mineral oil and gel solution were delivered by two independent syringe pumps during the coaxial electrospinning process. Subsequently, submicron bioactive glass tubes were obtained by removal of poly(vinyl pyrrolidone) and heavy mineral oil via calcination at 600°C for 5 h. Tubular structure was confirmed by scanning electron microscopy and transmission electron microscopy imaging. We examined the bioactivity of submicron bioactive glass tubes and fibers and evaluated their biocompatibility, using electrospun poly(-caprolactone) fibers - a bioinactive material - for comparison. The bioactivity of the glass tubes was examined in a simulated body fluid and they demonstrated the formation of hydroxyapatite-like minerals on both the outer and inner surfaces. In contrast, mineralization only occurred on their surface for bioactive glass solid fibers. Energy-dispersive X-ray data suggested that the bioactive glass tubes had a faster induction of mineral formation than the solid fibers. We demonstrate that the proliferation rate of mouse preosteoblastic MC3T3-E1 cells on bioactive glass tubes was comparable to that on solid fibers. We also show that bioactive glass tubes can be loaded with a model protein drug, bovine serum albumin, and that these structures exhibit delayed release properties. The bioactivity of released lysozyme can be as high as 90.9%. Taken together, these data suggest that submicron bioactive glass tubes could hold great potential for use in bone tissue engineering as well as topical drug or gene delivery.
机译：在这里，我们描述了一种使用溶胶-凝胶和同轴电纺技术制造亚微米生物活性玻璃管的方法，该技术可用于骨组织工程。在同轴静电纺丝过程中，重矿物油和凝胶溶液由两个独立的注射泵输送。随后，通过在600℃下煅烧5h除去聚乙烯基吡咯烷酮和重质矿物油，获得亚微米生物活性玻璃管。通过扫描电子显微镜和透射电子显微镜成像确认管状结构。我们使用电纺聚（己内酯）纤维（一种生物惰性材料）作了比较，检查了亚微米生物活性玻璃管和纤维的生物活性，并评估了它们的生物相容性。在模拟的体液中检查了玻璃管的生物活性，它们证明了在外表面和内表面上都形成了类似羟基磷灰石的矿物。相反，矿化仅发生在生物活性玻璃固体纤维的表面。能量色散X射线数据表明，生物活性玻璃管比固体纤维具有更快的矿物质形成诱导。我们证明了在生物活性玻璃管上的小鼠成骨前MC3T3-E1细胞的增殖速率与实心纤维上的相当。我们还表明，生物活性玻璃管可以装载模型蛋白药物，牛血清白蛋白，并且这些结构表现出延迟释放的特性。释放的溶菌酶的生物活性可以高达90.9％。综上所述，这些数据表明亚微米生物活性玻璃管在骨组织工程以及局部药物或基因递送中具有巨大的潜力。

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