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首页> 外文期刊>Acta biomaterialia >Autologous vs. allogenic mesenchymal progenitor cells for the reconstruction of critical sized segmental tibial bone defects in aged sheep.
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Autologous vs. allogenic mesenchymal progenitor cells for the reconstruction of critical sized segmental tibial bone defects in aged sheep.

机译：自体与同种异体间充质祖细胞用于重建老龄绵羊临界大小的胫骨节段骨缺损。

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摘要

Mesenchymal progenitor cells (MPCs) represent an attractive cell population for bone tissue engineering. Their special immunological characteristics suggest that MPCs may be used in allogenic applications. The objective of this study was to compare the regenerative potential of autologous vs. allogenic MPCs in an ovine critical size segmental defect model. Ovine MPCs were isolated from bone marrow aspirates, expanded and cultured with osteogenic medium for 2weeks before implantation. Autologous and allogenic transplantation was performed using the cell-seeded scaffolds and unloaded scaffolds, while the application of autologous bone grafts served as a control group (n=6). Bone healing was assessed 12weeks after surgery by radiology, microcomputed tomography, biomechanical testing and histology. Radiology, biomechanical testing and histology revealed no significant differences in bone formation between the autologous and allogenic groups. Both cell groups showed more bone formation than the scaffold alone, whereas the biomechanical data showed no significant differences between the cell groups and the unloaded scaffolds. The results of the study suggest that scaffold-based bone tissue engineering using allogenic cells offers the potential for an off-the-shelf product. Thus the results of this study serve as an important baseline for translation of the assessed concepts into clinical applications.
机译：间充质祖细胞（MPC）代表骨组织工程的有吸引力的细胞群。它们的特殊免疫学特征表明MPC可用于同种异体应用。这项研究的目的是在绵羊临界大小的节段性缺损模型中比较自体和同种MPC的再生潜力。从骨髓穿刺物中分离出绵羊MPC，进行扩增，并在植入前用成骨培养基培养2周。使用细胞接种的支架和空载的支架进行自体和同种异体移植，而使用自体骨移植物作为对照组（n = 6）。术后12周通过放射学，计算机断层扫描，生物力学测试和组织学评估骨愈合。放射学，生物力学测试和组织学显示，自体和同种异体组之间的骨形成没有显着差异。与单独的支架相比，两个细胞组均显示出更多的骨形成，而生物力学数据表明，在细胞组与未加载的支架之间没有显着差异。研究结果表明，使用同种异体细胞的基于支架的骨组织工程提供了现成产品的潜力。因此，这项研究的结果成为将评估概念转化为临床应用的重要基准。

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