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首页> 外文期刊>Acta biomaterialia >Acellular vascular grafts generated from collagen and elastin analogs
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Acellular vascular grafts generated from collagen and elastin analogs

机译：由胶原蛋白和弹性蛋白类似物产生的无细胞血管移植物

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摘要

Tissue-engineered vascular grafts require long fabrication times, in part due to the requirement of cells from a variety of cell sources to produce a robust, load-bearing extracellular matrix. Herein, we propose a design strategy for the fabrication of tubular conduits comprising collagen fiber networks and elastin-like protein polymers to mimic native tissue structure and function. Dense fibrillar collagen networks exhibited an ultimate tensile strength (UTS) of 0.71 ± 0.06 MPa, strain to failure of 37.1 ± 2.2% and Young's modulus of 2.09 ± 0.42 MPa, comparing favorably to a UTS and a Young's modulus for native blood vessels of 1.4-11.1 MPa and 1.5 ± 0.3 MPa, respectively. Resilience, a measure of recovered energy during unloading of matrices, demonstrated that 58.9 ± 4.4% of the energy was recovered during loading-unloading cycles. Rapid fabrication of multilayer tubular conduits with maintenance of native collagen ultrastructure was achieved with internal diameters ranging between 1 and 4 mm. Compliance and burst pressures exceeded 2.7 ± 0.3%/100 mmHg and 830 ± 131 mmHg, respectively, with a significant reduction in observed platelet adherence as compared to expanded polytetrafluoroethylene (ePTFE; 6.8 ± 0.05 × 10 5 vs. 62 ± 0.05 × 105 platelets mm -2, p 0.01). Using a rat aortic interposition model, early in vivo responses were evaluated at 2 weeks via Doppler ultrasound and CT angiography with immunohistochemistry confirming a limited early inflammatory response (n = 8). Engineered collagen-elastin composites represent a promising strategy for fabricating synthetic tissues with defined extracellular matrix content, composition and architecture.
机译：组织工程化的血管移植物需要较长的制造时间，部分原因是需要来自多种细胞来源的细胞来产生坚固的，承重的细胞外基质。在这里，我们提出了一种设计策略，用于制造包含胶原纤维网络和弹性蛋白样蛋白聚合物的管状导管，以模仿天然组织的结构和功能。致密的原纤维胶原蛋白网络表现出0.71±0.06 MPa的极限拉伸强度（UTS），37.1±2.2％的破坏应变和2.09±0.42 MPa的杨氏模量，与UTS和1.4的天然血管的杨氏模量相比具有优势-11.1 MPa和1.5±0.3 MPa。弹性是一种在矩阵卸载过程中回收的能量的度量，它表明在装载-卸载循环中回收了58.9±4.4％的能量。内径在1到4毫米之间，可以快速制造多层管状导管，并保持天然胶原的超微结构。顺应性和破裂压力分别超过2.7±0.3％/ 100 mmHg和830±131 mmHg，与膨胀聚四氟乙烯（ePTFE; 6.8±0.05×10 5与62±0.05×105血小板相比）观察到的血小板粘附性显着降低mm -2，p ＜0.01）。使用大鼠主动脉介入模型，在2周时通过多普勒超声和CT血管造影评估了早期体内反应，并通过免疫组织化学证实了有限的早期炎症反应（n = 8）。工程化的胶原蛋白-弹性蛋白复合材料代表了一种制造具有确定的细胞外基质含量，组成和结构的合成组织的有前途的策略。

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