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首页> 外文期刊>Acta biomaterialia >Gene delivery of PEI incorporating with functional block copolymer via non-covalent assembly strategy
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Gene delivery of PEI incorporating with functional block copolymer via non-covalent assembly strategy

机译：通过非共价组装策略整合功能嵌段共聚物的PEI基因的传递

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摘要

A novel functional diblock polymer P(PEGMA-b-MAH) is prepared and incorporated to improve the gene delivery efficiency of poly(ethyleneimine) PEI via non-covalent assembly strategy. First, P(PEGMA-b-MAH) is prepared from l-methacrylamidohistidine methyl ester (MAH) by reversible addition fragmentation chain transfer polymerization, with poly[poly(ethylene glycol) methyl ether methacrylate] (P(PEGMA)) as the macroinitiator. Then P(PEGMA-b-MAH) is assembled with plasmid DNA (pDNA) and PEI (Mw = 10 kDa) to form PEI/P(PEGMA-b-MAH)/pDNA ternary complexes. The agarose gel retardation assay shows that the presence of P(PEGMA-b-MAH) does not interfere with DNA condensation by the PEI. Dynamic light scattering tests show that PEI/P(PEGMA-b-MAH)/pDNA ternary complexes have excellent serum stability. In vitro transfection indicates that, compared to the P(PEGMA-b-MAH) free PEI-25k/pDNA binary complexes, PEI-10k/P(PEGMA-b-MAH)/pDNA ternary complexes have lower cytotoxicity and higher gene transfection efficiency, especially under serum conditions. The ternary complexes proposed here can inspire a new strategy for the development of gene and drug delivery vectors. ? 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
机译：制备并掺入新颖的功能性双嵌段聚合物P（PEGMA-b-MAH），以通过非共价组装策略提高聚（乙烯亚胺）PEI的基因递送效率。首先，以聚[聚（乙二醇）甲基醚甲基丙烯酸酯]（P（PEGMA））为大分子引发剂，通过可逆加成断裂链转移聚合反应，由1-甲基丙烯酰胺基组氨酸甲酯（MAH）制备P（PEGMA-b-MAH）。 。然后将P（PEGMA-b-MAH）与质粒DNA（pDNA）和PEI（Mw = 10 kDa）组装在一起，形成PEI / P（PEGMA-b-MAH）/ pDNA三元复合物。琼脂糖凝胶阻滞试验表明P（PEGMA-b-MAH）的存在不干扰PEI引起的DNA缩合。动态光散射测试表明，PEI / P（PEGMA-b-MAH）/ pDNA三元复合物具有出色的血清稳定性。体外转染表明，与不含P（PEGMA-b-MAH）的PEI-25k / pDNA二元复合物相比，PEI-10k / P（PEGMA-b-MAH）/ pDNA三元复合物具有较低的细胞毒性和较高的基因转染效率，尤其是在血清条件下。本文提出的三元复合物可以为基因和药物递送载体的开发激发新的策略。 ？ 2012年Acta Materialia Inc.由Elsevier Ltd.发行。保留所有权利。

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