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首页> 外文期刊>Acta biomaterialia >Macroporous and nanofibrous hyaluronic acid/collagen hybrid scaffold fabricated by concurrent electrospinning and deposition/leaching of salt particles.
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Macroporous and nanofibrous hyaluronic acid/collagen hybrid scaffold fabricated by concurrent electrospinning and deposition/leaching of salt particles.

机译：通过同时静电纺丝和盐颗粒的沉积/浸提制备的大孔和纳米纤维透明质酸/胶原杂化支架。

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摘要

A three-dimensional (3-D) macroporous and nanofibrous hyaluronic acid (HA) scaffold was fabricated by an electrospinning process combined with a salt leaching technique. HA and collagen were dissolved in a sodium hydroxide (NaOH)/N,N-dimethyl formamide (DMF) solvent mixture at a concentration of 10 wt.% and successfully electrospun into a nanofiber web with a soft, fluffy structure by the combined effects of numerous minijet evolutions and their subsequent vertical growth. To our knowledge, the formation of an extensive fluffy nanofiber morphology is the first time as a single route has been used to spontaneously generate a 3-D nanofibrous structure. By the simultaneous deposition of salt particulates as a porogen during electrospinning and subsequent chemical cross-linking and salt leaching, a water-swellable HA-based scaffold retaining a macroporous and nanofibrous geometry could be produced. Bovine chondrocytes were cultured on the HA/collagen scaffold to assessing the scaffold's cytocompatibility. The results revealed that cellular adhesion and proliferation were enhanced in proportion to the content of collagen, and the seeded chondrocytes maintained the roundness characteristic of a chondroblastic morphology.
机译：通过电纺丝工艺结合盐浸技术制备了三维（3-D）大孔和纳米纤维状透明质酸（HA）支架。将HA和胶原蛋白以10 wt。％的浓度溶于氢氧化钠（NaOH）/ N，N-二甲基甲酰胺（DMF）溶剂混合物中，并通过以下方法的联合作用成功地电纺成具有柔软蓬松结构的纳米纤维网微型喷气机的众多发展及其随后的垂直增长。据我们所知，广泛的蓬松纳米纤维形态的形成是第一次，因为已经使用单一途径自发产生3-D纳米纤维结构。通过在静电纺丝过程中同时沉积盐颗粒作为致孔剂，随后进行化学交联和盐浸，可以生产出具有大孔和纳米纤维几何形状的水溶性HA基支架。在HA /胶原蛋白支架上培养牛软骨细胞，以评估支架的细胞相容性。结果表明，细胞粘附和增殖与胶原含量成比例地增加，并且播种的软骨细胞保持了软骨细胞形态的圆度特征。

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