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首页> 外文期刊>Acta biomaterialia >Calorimetric study of extracellular tissue matrix degradation and instability after gamma irradiation.
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Calorimetric study of extracellular tissue matrix degradation and instability after gamma irradiation.

机译：γ射线照射后细胞外组织基质降解和不稳定性的量热研究。

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摘要

Native extracellular tissue matrix (ECM) is increasingly used for tissue repair and regeneration. The kinetics of gamma irradiation damage on human dermis ECM was studied by differential scanning calorimetry (DSC). Dermis ECM was irradiated at a low-dose rate of 0.23kGyh(-1) in order to study the progression of ECM damage as the gamma dose increased from 0 to 32kGy. The study showed that the effect of gamma irradiation above 2kGy was predominantly peptide chain scission. As the gamma dose increased, the stability of irradiated ECM decreased further, and multiple ECM domains of different stability were detected. Even a moderate gamma dose (7-12kGy) could decrease the onset denaturation temperature of ECM to below body temperature. DSC analysis also showed partial and spontaneous protein denaturation in gamma-irradiated, rehydrated ECM at 37 degrees C. In vitro rehydration tests confirmed that a significant fraction of the irradiated ECM disintegrated into minute ECM fragments at 37 degrees C, although the irradiated ECM appeared to be normal at 4 degrees C and room temperature. DSC data were correlated well to effects of gamma irradiation on ECM microstructure, mechanical property and in vitro cell response reported earlier by us. A model was presented to describe the kinetics of gamma-irradiation-induced alterations of tissue ECM properties.
机译：天然细胞外组织基质（ECM）越来越多地用于组织修复和再生。通过差示扫描量热法（DSC）研究了γ射线对人真皮ECM损伤的动力学。为了研究低剂量0.23kGyh（-1）照射的真皮ECM，以研究随着γ剂量从0增加到32kGy，ECM损伤的进展。研究表明，高于2kGy的伽马射线辐射主要是肽链断裂。随着γ剂量的增加，辐射的ECM的稳定性进一步降低，并且检测到多个具有不同稳定性的ECM域。即使适度的伽玛剂量（7-12kGy）也可以将ECM的起始变性温度降低至体温以下。 DSC分析还显示了在37摄氏度下经伽马射线辐照，再水化的ECM中蛋白质的部分和自发变性。体外再水化试验证实，在37摄氏度下，很大一部分辐照的ECM分解成细小的ECM碎片，尽管辐照的ECM似乎能在4摄氏度和室温下正常。 DSC数据与γ射线对ECM的微观结构，机械性能和我们先前报道的体外细胞反应的影响密切相关。提出了一个模型来描述γ射线诱导的组织ECM特性改变的动力学。

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