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首页> 外文期刊>RSC Advances >Magnetic iron oxide nanoparticles encapsulating horseradish peroxidase (HRP): synthesis, characterization and carrier for the generation of free radicals for potential applications in cancer therapy
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Magnetic iron oxide nanoparticles encapsulating horseradish peroxidase (HRP): synthesis, characterization and carrier for the generation of free radicals for potential applications in cancer therapy

机译：磁性氧化铁纳米粒子包封辣根过氧化物酶（HRP）：用于产生自由基的合成，表征和载体，用于癌症治疗中的潜在应用

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摘要

This article reports a method of preparation of iron oxide nanoparticles using a reverse micellar (water-in-oil) approach. We have encapsulated horseradish peroxidase (HRP) in iron oxide nanoparticles. HRTEM, XRD, and DLS analyses showed that the average diameter of these particles was around 20 nm, 20.5 nm, and 30 nm, respectively, and the particles were highly monodispersed with spherical morphology. The entrapment efficiency of HRP was found to be as high as 92%. Practically, the entrapped enzyme shows zero leachability for up to 30 days. Enzyme entrapped in iron oxide nanoparticles followed MichaelisMenten kinetics and showed higher stability towards temperature change as compared to free enzyme. Entrapped enzyme is stable at up to 65 degrees C; however, the free enzyme starts to lose its activity above 38 degrees C. The entrapped enzyme, HRP, has been used to convert a benign prodrug, indole-3-acetic acid (IAA), to a toxic oxidized product, and its toxic effect has been tested on cancerous cell lines through thiazolyl blue tetrazolium blue (MTT) assay. MTT assay on two cancer cell lines revealed that indole acetic acid (IAA), the prodrug alone, had no cytotoxic effect, and it became active only after oxidative decarboxylation by HRP. The benign substrate IAA reaches the cells and is oxidized by HRP. IAA, on reacting with HRP, forms free radicals such as indolyl, skatole and peroxyl radicals. This creates severe oxidative stress in the cancer cells, resulting in cell death.
机译：本文举报了一种使用反向胶束（油油）方法制备氧化铁纳米颗粒的方法。我们在氧化铁纳米粒子中封装了辣根过氧化物酶（HRP）。 HRTEM，XRD和DLS分析表明，这些颗粒的平均直径分别为约20nm，20.5nm和30nm，颗粒高度单分散，具有球形形态。发现HRP的血管效率高达92％。实际上，捕获的酶显示零可浸出性长达30天。诱捕氧化铁纳米粒子的酶洗涤迈克利灭菌动力学，与游离酶相比，朝向温度变化的稳定性更高。捕获的酶在高达65摄氏度上稳定;然而，自由酶开始失去其高于38摄氏度的活性。捕获的酶HRP已被用于将良性前药，吲哚-3-乙酸（IAA）转化为有毒氧化产物及其毒性效应已经通过噻唑基蓝四唑鎓蓝色（MTT）测定对癌细胞系进行了测试。 MTT测定在两种癌细胞系上显示吲哚乙酸（IAA），单独的前药，没有细胞毒性作用，并且仅在通过HRP氧化脱羧后的活性。良性底物IAA达到细胞并通过HRP氧化。在与HRP反应的情况下，IAA形成自由基，例如吲哚基，石棉和过氧基。这在癌细胞中产生了严重的氧化胁迫，导致细胞死亡。

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  • 来源
    《RSC Advances》 |2016年第112期|共10页
  • 作者

    Gupta Nikesh; Gupta Chetna; Sharma Sandeep; Rathi Brijesh; Sharma Rakesh Kumar; Bohidar H. B.;

  • 作者单位

    Jawaharlal Nehru Univ Special Ctr Nanosci SCNS New Delhi 110067 India;

    Univ Delhi Hansraj Coll Dept Chem Delhi 110007 India;

    Univ Delhi Dept Chem Nanotechnol Drug Delivery &

    Tissue Culture Res La Delhi 110007 India;

    Univ Delhi Hansraj Coll Dept Chem Delhi 110007 India;

    Univ Delhi Dept Chem Nanotechnol Drug Delivery &

    Tissue Culture Res La Delhi 110007 India;

    Jawaharlal Nehru Univ Special Ctr Nanosci SCNS New Delhi 110067 India;

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  • 正文语种 eng
  • 中图分类 化学;
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