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首页> 外文期刊>RSC Advances >A rapid spectrofluorimetric method for the determination of malondialdehyde in human plasma after its derivatization with thiobarbituric acid and vortex assisted liquid-liquid microextraction
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A rapid spectrofluorimetric method for the determination of malondialdehyde in human plasma after its derivatization with thiobarbituric acid and vortex assisted liquid-liquid microextraction

机译：一种快速的光谱氟化物方法，用于测定硫酸乙酸和涡旋辅助液 - 液微萃取后衍生化后人血浆中丙二醛的测定

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摘要

A rapid, simple and sensitive methodology has been developed for the determination of trace levels of malondialdehyde (MDA) in human plasma. The method is based on the derivatization of MDA using thiobarbituric acid (TBA) to form a highly fluorescent compound, "MDA-TBA" at acidic pH. The TBA-derivatized MDA formed was preconcentrated using vortex assisted liquid-liquid microextraction into 90 mu L of n-heptanol as extractant solvent and measured spectro-fluorimetrically. Parameters such as nature of the extraction solvent, extraction time, sample volume, temperature, derivatization reaction time and pH of the sample solution were studied and optimized. The developed protocol was found to yield a linear calibration curve in the concentration range between 1.0-100.0 mu g L-1 with a limit of detection of 0.32 mu g L-1. The enrichment factor for the analyte was calculated to be 65. The repeatability of the method was satisfactory (RSD <= 1.6%). Total extraction time was less than 10 min. The developed method was successfully applied to the determination of MDA in a human plasma sample.
机译：已经开发了一种快速，简单敏感的方法，用于测定人血浆中丙醛（MDA）的痕量水平。该方法基于MDA的衍生化使用硫酰比呋喃酸（TBA）形成高度荧光化合物，在酸性pH下“MDA-TBA”。将形成的TBA衍生的MDA使用涡旋辅助液 - 液微萃取至90μl正庚醇作为萃取剂溶剂并测量光谱 - 氟互氧化物。研究并优化了诸如提取溶剂，提取时间，样品体积，温度，衍生化反应时间和pH的萃取溶剂，提取时间，样品体积，温度，衍生反应时间和pH的参数。发现开发的方案在1.0-100.0μgl-1之间的浓度范围内产生线性校准曲线，其检测为0.32μgl-1。计算分析物的富集因子为65.该方法的可重复性令人满意（RSD <= 1.6％）。总提取时间小于10分钟。开发方法已成功应用于人血浆样品中MDA的测定。

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  • 来源
    《RSC Advances》 |2016年第3期|共7页
  • 作者

    Yahyavi Hossain; Kaykhaii Massoud; Hashemi Mohammad;

  • 作者单位

    Univ Sistan &

    Baluchestan Dept Chem Fac Sci Zahedan 98135674 Iran;

    Univ Sistan &

    Baluchestan Dept Chem Fac Sci Zahedan 98135674 Iran;

    Zahedan Univ Med Sci Cellular Mol Res Ctr Dept Clin Biochem Zahedan Iran;

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  • 正文语种 eng
  • 中图分类 化学;
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