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首页> 外文期刊>RSC Advances >Preparation and characterization of biocompatible molecularly imprinted poly(ionic liquid) films on the surface of multi-walled carbon nanotubes
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Preparation and characterization of biocompatible molecularly imprinted poly(ionic liquid) films on the surface of multi-walled carbon nanotubes

机译：多壁碳纳米管表面的生物相容性分子印迹聚（离子液）膜的制备与表征

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摘要

A series of novel protein molecularly imprinted polymers (MIPs) were synthesized through a surface molecular imprinting technique by using bovine serum albumin (BSA) as a template, acrylamide modified multi-walled carbon nanotubes (MWCNTs-AAm) as substrates, and allyl-functionalized ionic liquids (ILs) as monomers. The MWCNTs@BSA-MIPILs synthesized under different monomer/template/cross-linker ratios were characterized by FTIR, SEM and BET analyses. The adsorption kinetics and isotherm, imprinting effect, selectivity and competitiveness, reusability, and practical applicability were evaluated in detail. The influence of the outside diameters of MWCNTs and the anion types of ILs on the imprinting effect of the MWCNTs@BSA-MIPILs was also studied. The MWCNTs with smaller diameters (<8 nm and 10-20 nm) were more beneficial for the preparation of the surface imprinted polymers. The MIPs prepared with IL monomers composed of anions with low nucleophilicity and hydrogen bond basicity (PF6-), and high steric effect (CF3SO3-) were found to have a better imprinting effect in comparison with those prepared with Cl- and BF4--based IL and the traditional acrylamide monomers. They also exhibited higher selective recognition ability for BSA than for human serum albumin, lysozyme, trypsin and bovine hemoglobin. The imprinting and selectivity factors were greatly improved in a binary protein solution containing BSA and bovine hemoglobin. The developed MWCNTs@BSA-MIPILs were also successfully used for the purification of BSA from bovine calf serum. These results indicated that the PF6- and CF3SO3--based ILs, for their important role in stabilizing biomacromolecules, will be ideal functional monomers for the development of biocompatible MIPs for protein molecules.
机译：一系列新的蛋白质分子印迹聚合物（MIP）的通过使用牛血清白蛋白（BSA）作为模板，丙烯酰胺改性的多壁碳纳米管（MWCNT-AAM）作为底物，和烯丙基官能化通过表面分子印迹技术合成离子液体（离子液体）作为单体。通过FTIR，SEM和BET分析多壁碳纳米管@下不同的单体/模板/交联剂比率合成的BSA-MIPILs进行了表征。吸附动力学和等温线，压印效果，选择性和竞争力，可重用性，和实用性进行了详细的评价。多壁碳纳米管和阴离子类型的多壁碳纳米管@ BSA-MIPILs的压印效果离子液体的外径的影响也进行了研究。具有较小直径（<8纳米和10-20纳米）多壁碳纳米管是用于表面印迹聚合物的制备中更为有利。与低亲核性和氢键碱度（PF6-），和高的空间位阻效应（CF3SO3-）阴离子构成的IL单体制备了MIPS被发现具有与那些具有Cl-和BF 4制备比较更好的压印效果 - 根据IL和传统的丙烯酰胺单体。它们还表现出比对人血清白蛋白，溶菌酶，胰蛋白酶和牛血红蛋白为BSA更高选择性识别能力。压印和选择性的因素在含有BSA和牛血红蛋白的二进制蛋白质溶液中大大提高。所开发的多壁碳纳米管@ BSA-MIPILs也成功地用于BSA的来自小牛血清纯化。这些结果表明，PF 6 - 和CF 3 SO 3 - 的离子液体基，它们在稳定生物大分子重要的作用，将是生物相容的MIP的发展，为蛋白质分子理想官能单体。

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  • 来源
    《RSC Advances》 |2016年第49期|共13页
  • 作者

    Ding Haoyuan; Chen Rongfeng; Liu Mingming; Huang Rong; Du Yamei; Huang Chao; Yu Xiaoyang; Feng Xionghan; Liu Fan;

  • 作者单位

    Huazhong Agr Univ Key Lab Arable Land Conservat Middle &

    Lower Reac Minist Agr Coll Resources &

    Environm Wuhan 430070 Peoples R China;

    Huazhong Agr Univ Key Lab Arable Land Conservat Middle &

    Lower Reac Minist Agr Coll Resources &

    Environm Wuhan 430070 Peoples R China;

    Huazhong Agr Univ Key Lab Arable Land Conservat Middle &

    Lower Reac Minist Agr Coll Resources &

    Environm Wuhan 430070 Peoples R China;

    Huazhong Agr Univ Key Lab Arable Land Conservat Middle &

    Lower Reac Minist Agr Coll Resources &

    Environm Wuhan 430070 Peoples R China;

    Huazhong Agr Univ Key Lab Arable Land Conservat Middle &

    Lower Reac Minist Agr Coll Resources &

    Environm Wuhan 430070 Peoples R China;

    Huazhong Agr Univ Key Lab Arable Land Conservat Middle &

    Lower Reac Minist Agr Coll Resources &

    Environm Wuhan 430070 Peoples R China;

    Huazhong Agr Univ Key Lab Arable Land Conservat Middle &

    Lower Reac Minist Agr Coll Resources &

    Environm Wuhan 430070 Peoples R China;

    Huazhong Agr Univ Key Lab Arable Land Conservat Middle &

    Lower Reac Minist Agr Coll Resources &

    Environm Wuhan 430070 Peoples R China;

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