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Biophysical insight into the interaction of the bioflavonoid kaempferol with triple and double helical RNA and the dual fluorescence behaviour of kaempferol

机译：生物物理洞察生物剥糖kaempferol与三重双螺旋RNA的相互作用及Kaempferol的双重荧光行为

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摘要

The interaction of the naturally occurring flavonoid, kaempferol (KMP) with single, double and triple helical forms of RNA has been investigated by different spectroscopic and viscometric techniques. It was found that KMP binds with triple helical [poly(U).poly(A)* poly(U), hereafter U.A*U, the dot represents the Watson-Crick and asterisk represents Hoogsteen base pairing respectively] and double helical [poly(A). poly(U), hereafter A. U] forms of RNA, whereas no interaction was observed with single stranded polyuridylic acid [poly-U] under identical experimental conditions. The binding of KMP was found to be stronger with U.A*U (20 x 10(4) M-1) compared to that of the parent duplex A. U (9.5 x 10(4) M-1). From the Stern-Volmer quenching constant, viscosity measurement and perturbation of CD spectra of RNA revealed that KMP binds to the U.A*U structure by intercalation while partial intercalation has been proposed for the binding to the duplex RNA structure. Thermodynamic data obtained from the temperature dependence study showed that the association was favoured by negative enthalpy and positive entropy changes. Experimental observations indicated that KMP binds and stabilizes the RNA-triplex more than its parent duplex counterpart.
机译：通过不同的光谱和粘度技术研究了天然存在的类黄酮，kaempferol（Kaempferol（Kmp）与单一，双螺旋形式的RNA的相互作用。发现kmp与三螺旋[聚（u）.poly（a）* poly（u），下文ua * u，点表示Watson-Crick和Asterisk分别代表Hoogsteen底座配对]和双螺旋[Poly （一种）。 poly（U），下文中A. U] RNA的形式，而在相同的实验条件下，用单链聚脲酰基[Poly-U]没有观察到相互作用。与父双相A. U（9.5 x 10（4）M-1）相比，发现KMP的结合与U.a * U（20×10（4）m-1）更强。从船尾淬火恒定，RNA的CD光谱的粘度测量和扰动显示KMP通过插入嵌入与U.a * U结构结合，而已经提出了与双相RNA结构结合的部分插层。从温度依赖性研究中获得的热力学数据表明，该关联受阴性焓和正熵变化的青睐。实验观察结果表明，KMP结合并稳定了比其母体双相对应的RNA三重交流。

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  • 来源
    《RSC Advances》 |2016年第87期|共12页
  • 作者

    Haque Lucy; Bhuiya Sutanwi; Tiwari Richa; Pradhan Ankur Bikash; Das Suman;

  • 作者单位

    Jadavpur Univ Dept Chem Raja SC Mullick Rd Kolkata 700032 India;

    Jadavpur Univ Dept Chem Raja SC Mullick Rd Kolkata 700032 India;

    Jadavpur Univ Dept Chem Raja SC Mullick Rd Kolkata 700032 India;

    Jadavpur Univ Dept Chem Raja SC Mullick Rd Kolkata 700032 India;

    Jadavpur Univ Dept Chem Raja SC Mullick Rd Kolkata 700032 India;

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  • 正文语种 eng
  • 中图分类 化学;
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