• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >A ratiometric fluorescent probe for quantification of alkaline phosphatase in living cells
【24h】

A ratiometric fluorescent probe for quantification of alkaline phosphatase in living cells

机译：用于定量活细胞碱性磷酸酶的比例荧光探针

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A ratiometric fluorescent probe with strong intramolecular charge transfer (ICT) character has been designed for the detection of alkaline phosphatase (ALP). In the presence of ALP, probe 1 showed a dramatic bathochromic shift from 550 to 650 nm in the fluorescence spectrum and an obvious ratiometric signal with an isoemissive point was observed. The spectral change was attributed to the hydrolysis and cleavage of a phosphate group from the probe that changed the ability of the intramolecular charge transfer. The fluorescence intensity ratio displayed a linear relationship against the concentration of ALP in the concentration range from 50 to 200 U L-1. Other biological species, including lysozyme, trypsin, pepsin, acetyl cholinesterase, carboxylesterase, and bovine serum albumin, did not induce distinct spectral changes of the probe, indicating its selective sensing ability to ALP. Furthermore, probe 1 was also successfully applied to image endogenous ALP activity in living cells.
机译：具有强大的分子内电荷转移（ICT）特征的比率荧光探针专为检测碱性磷酸酶（ALP）。 在ALP的存在下，探针1显示荧光光谱中的550至650nm的显着碱性偏振偏移，并且观察到具有异种点的明显的比例信号。 光谱变化归因于来自探针的磷酸盐基团的水解和切割，改变分子内电荷转移的能力。 荧光强度比以50至200μl1-1的浓度范围内的ALP浓度显示出直线关系。 其他生物物种，包括溶菌酶，胰蛋白酶，胃蛋白酶，乙酰胆碱酯酶，羧酸酯酶和牛血清白蛋白并未诱导探针的不同光谱变化，表明其对ALP的选择性感测能力。 此外，探针1也成功地应用于活细胞中的图像内源性ALP活性。

著录项

  • 来源
    《RSC Advances》 |2016年第38期|共6页
  • 作者

    Lu Zhenxing; Wu Jiasheng; Liu Weimin; Zhang Guangyou; Wang Pengfei;

  • 作者单位

    Chinese Acad Sci Tech Inst Phys &

    Chem Key Lab Photochem Convers &

    Optoelect Mat Beijing 100190 Peoples R China;

    Chinese Acad Sci Tech Inst Phys &

    Chem Key Lab Photochem Convers &

    Optoelect Mat Beijing 100190 Peoples R China;

    Chinese Acad Sci Tech Inst Phys &

    Chem Key Lab Photochem Convers &

    Optoelect Mat Beijing 100190 Peoples R China;

    Univ Jinan Sch Chem &

    Chem Engn Jinan 250022 Peoples R China;

    Chinese Acad Sci Tech Inst Phys &

    Chem Key Lab Photochem Convers &

    Optoelect Mat Beijing 100190 Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号